Chromatin – DNA fragments by sonication and immunoprecipitated
chipready

Protocol for Recovery of Bacterial DNA From Water Filters

Zoe
Zoe

Improved protocol for recovery of bacterial DNA from water filters: Sonication and backflushing of commercial syringe filters.

Extraction of bacterial DNA from water filters is frequently used in aquatic microbial ecology, e.g., for sequencing-based diversity studies. Here, we describe an improved size-class filtration and bacterial DNA extraction protocol using commercial “syringe filters”, involving sonication- and backflushing-steps and DNA-binding magnetic beads. Importantly, the sonication-step improved DNA recovery up to 10-fold. The DNA extraction-turnaround time is 4h, with a hands-on time of about 2h.

Fragmentation of DNA in a sub-microliter microfluidic sonication device.

Fragmentation of DNA is an essential step for many biological applications including the preparation of next-generation sequencing (NGS) libraries. As sequencing technologies push the limits towards single cell and single molecule resolution, it is of great interest to reduce the scale of this upstream fragmentation step.

 

Here we describe a miniaturized DNA shearing device capable of processing sub-microliter samples based on acoustic shearing within a microfluidic chip. A strong acoustic field was generated by a Langevin-type piezo transducer and coupled into the microfluidic channel via the flexural lamb wave mode.

 

Purified genomic DNA, as well as covalently cross-linked chromatin were sheared into various fragment sizes ranging from ∼180 bp to 4 kb. With the use of standard PDMS soft lithography, our approach should facilitate the integration of additional microfluidic modules and ultimately allow miniaturized NGS workflows

Fragmentation of DNA by sonication.

INTRODUCTIONDNA fragmentation is often necessary prior to library construction or subcloning for DNA sequencing. This protocol describes a method for DNA fragmentation by sonication. During sonication, DNA samples are subjected to hydrodynamic shearing by exposure to brief periods of sonication.

DNA that has been sonicated for excessive periods of time is extremely difficult to clone. Most sonicators will not shear DNA to a size of less than 300-500 bp, and it is tempting to continue sonication until the entire DNA population has been reduced in size. However, the yield of subclones is usually greater if sonication is stopped when the fragments of the target DNA first reach a size of ~700 bp.

 

chipready
chipready

Streptavidin Magnetic Beads

HY-K0208 5 mL
EUR 645.6

Anti-HA magnetic beads

B26201 1 mL
EUR 579.6
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

Anti-HA magnetic beads

B26202 5 mL
EUR 1742.4
Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification.

Anti-HA Magnetic Beads

HY-K0201 1 mL
EUR 334.8

Anti-Myc magnetic beads

B26301 1 mL
EUR 579.6
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Anti-Myc magnetic beads

B26302 5 mL
EUR 1742.4
Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification.

Protein A Magnetic Beads

6507-1 each
EUR 314.4

Protein G Magnetic Beads

6517-1 each
EUR 314.4

Protein L Magnetic Beads

6537-1 each
EUR 352.8

Protein A Magnetic Beads

HY-K0203 1 mL
EUR 148.8

Protein G Magnetic Beads

HY-K0204 5 mL
EUR 445.2

Anti-Flag magnetic beads

B26101 1 mL
EUR 579.6
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

Anti-Flag magnetic beads

B26102 5 mL
EUR 1742.4
Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification.

Anti-Flag Magnetic Beads

HY-K0207 1 mL
EUR 570

BlackPearl® (Magnetic Beads)

20161006-1 - Ask for price

BlackPearl® (Magnetic Beads)

20161006-2 - Ask for price

BlackPearl® (Magnetic Beads)

20161006-3 - Ask for price

Magnetic Beads™ Streptavidin

SMB-B01 5mg
EUR 246.1
Description: The Streptavidin-Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin (SA). The beads can be used to capture the biotinylated proteins or other molecules, because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly.Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidin, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).The Streptavidin-Magnetic Beads is easy to capture the biotinylated proteins or other molecules in Chemiluminescence procedures, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Protein A/G Magnetic Beads

6527-1 each
EUR 352.8

Protein A/G Magnetic Beads

HY-K0202 1 mL
EUR 157.2

Anti-c-Myc Magnetic Beads

HY-K0206 5 mL
EUR 1368

Anti-HA (Nanobody) Magnetic Beads

AE109 500μL
EUR 403

Anti-GFP (Nanobody) Magnetic Beads

AE079 500μL
EUR 312

Anti-Myc (Nanobody) Magnetic Beads

AE107 1000μL
EUR 213.2

Protein A/G/L Magnetic Beads

6547-1 each
EUR 405.6

Human CD33-coupled Magnetic Beads

MBC-K008 2mg
EUR 2707.1
Description: Human CD33 protein is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259(Accession # P20138-1(R69G)).

Human CD38-coupled Magnetic Beads

MBC-K010 2mg
EUR 2707.1
Description: Human CD38 protein is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300(Accession # P28907-1).

Human EGFR-coupled Magnetic Beads

MBE-K012 2mg
EUR 2707.1
Description: Human EGFR protein is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645(Accession # P00533-1).

Human PSMA-coupled Magnetic Beads

MBP-K007 2mg
EUR 3349.1
Description: Human PSMA protein is expressed from human 293 cells (HEK293). It contains AA Lys 44 - Ala 750(Accession # Q04609-1).

Human ROR1-coupled Magnetic Beads

MBR-K009 2mg
EUR 2707.1
Description: Human ROR1 protein is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403(Accession # Q01973-1).

Human BCMA-coupled Magnetic Beads

MBS-K004 2mg
EUR 2707.1
Description: Human BCMA protein is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54(Accession # Q02223-1).

Human CD19-coupled Magnetic Beads

MBS-K005 2mg
EUR 4312.1
Description: Human CD19 protein is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291(Accession # P15391-1).

Human HER2-coupled Magnetic Beads

MBS-K006 500ug
EUR 2835.5
Description: Human HER2 protein is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652(Accession # P04626-1).

Human ACE2-coupled Magnetic Beads

MBS-K013 2mg
EUR 2707.1
Description: Human ACE2 protein is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740(Accession # Q9BYF1-1).

Human CD73-coupled Magnetic Beads

MBS-K022 2mg
EUR 3349.1
Description: Human CD73 Protein is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549(Accession # P21589-1).

Human CD47-coupled Magnetic Beads

MBS-K023 2mg
EUR 2707.1
Description: Human CD47 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139(Accession # Q08722).

Human CD3E-coupled Magnetic Beads

MBS-K026 2mg
EUR 2707.1
Description: Human CD3E Protein is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126(Accession # P07766-1).

Human CD22-coupled Magnetic Beads

MBS-K052 2mg
EUR 4312.1
Description: Human CD22 Protein is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687(Accession # P20273-1).

Human DLL3-coupled Magnetic Beads

MBS-K053 2mg
EUR 2707.1
Description: Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.

Human uPAR-coupled Magnetic Beads

MBS-K076 2mg
EUR 2707.1
Description: Tumors are the result of uncontrolled proliferation of cells in different organs. Tumor development is a multistage process, that includes the generation of primary tumors, separation of tumors from primary sites, degradation of extracellular matrix (ECM), and distant metastasis of tumors. A variety of genes play important roles in the development of tumors, including the cell surface receptor urokinase-type plasminogen activator receptor (uPAR). uPAR is highly expressed in a variety of tumor cells, and a variety of signals regulated by uPAR play significant roles in tumor cell proliferation and metastasis, tumor-related glycolysis, the tumor microenvironment and angiogenesis. Studies have found that some specific drugs and antibodies have unique inhibitory effects on uPAR.

Human PCSK9-coupled Magnetic Beads

MBS-K054 2mg
EUR 2707.1
Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Human ASGR1-coupled Magnetic Beads

MBS-K055 2mg
EUR 2707.1
Description: ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2). ASGR1 has been shown to be efficiently targeted to the plasma membrane and to undergo constitutive endocytosis and recycling and found mainly expressed in the human liver.

Amylose BlackPearl® (Magnetic Beads)

20161009-1 - Ask for price

Amylose BlackPearl® (Magnetic Beads)

20161009-2 - Ask for price

Human ANGPT2-coupled Magnetic Beads

MBS-K035 2mg
EUR 2707.1
Description: Human ANGPT2 Protein is expressed from human 293 cells (HEK293). It contains AA Lys 275 - Phe 496(Accession # O15123-1).

Human VEGF165-coupled Magnetic Beads

MBS-K036 2mg
EUR 2707.1
Description: Human VEGF165 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191(Accession # P15692-4).

Human EGFRvIII-coupled Magnetic Beads

MBS-K020 2mg
EUR 2707.1
Description: Human EGFRvIII Protein is expressed from human 293 cells (HEK293). It contains AA Leu 25- Ser 378(Accession # NP_001333870.1).

Human HGF R-coupled Magnetic Beads

MBS-K021 2mg
EUR 2707.1
Description: Human HGF R Protein is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932(Accession # P08581).

Human CTLA-4 coupled Magnetic Beads

MBS-K042 2mg
EUR 2707.1
Description: Human CTLA-4 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162(Accession # Q6GR94-1).

Human Mesothelin-coupled Magnetic Beads

MBS-K024 2mg
EUR 2707.1
Description: Human Mesothelin Protein is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580(Accession # Q13421-3(R309P)).

Streptavidin BlackPearl® (Magnetic Beads)

20161004-1 - Ask for price

Streptavidin BlackPearl® (Magnetic Beads)

20161004-2 - Ask for price

Protein A/G Magnetic Beads for IP

B23201 1 ml
EUR 158.4
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Protein A/G Magnetic Beads for IP

B23202 5 ml
EUR 558
Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens.

Human TROP-2-coupled Magnetic Beads

MBS-K028 2mg
EUR 2707.1
Description: Human TROP-2 Protein is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274(Accession # P09758-1).

Protein A BlackPearl® (Magnetic Beads)

20161000-1 - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-2 - Ask for price

Protein A BlackPearl® (Magnetic Beads)

20161000-3 - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-1 - Ask for price

Protein G BlackPearl® (Magnetic Beads)

20161003-2 - Ask for price

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-1 - Ask for price

Nickel (Ni2+) BlackPearl® (Magnetic Beads)

20161010-2 - Ask for price

Human Nectin-4-coupled Magnetic Beads

MBS-K039 2mg
EUR 2707.1
Description: Human Nectin-4 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349(Accession # Q96NY8-1).

Anti-mCherry (Nanobody) Magnetic Beads Ab

AE094 1000μL
EUR 213.2

Human B7-H3(4Ig)-coupled Magnetic Beads

MBS-K040 2mg
EUR 2707.1
Description: Human B7-H3 Protein is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461(Accession # Q5ZPR3-1).

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-1 - Ask for price

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-2 - Ask for price

Calmodulin (CAM) BlackPearl® (Magnetic Beads)

20161002-3 - Ask for price

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-1 - Ask for price

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-2 - Ask for price

Glutathione (GSH) BlackPearl® (Magnetic Beads)

20161007-3 - Ask for price

Universal IP/Co-IP Toolkit (Magnetic Beads)

KTD104-EN-20T 20 T
EUR 169
Description: Antibody coupling magnetic beads, suitable for IP/ CO-IP experiment, protein purification.

PE-labeled Human CD47-coupled Magnetic Beads

MBS-K059 2.5mg
EUR 4536.8
Description: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

APC-labeled Human CD47-coupled Magnetic Beads

MBS-K060 0.5mg
EUR 3028.1
Description: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

SARS-CoV-2 Spike S1-coupled Magnetic Beads

MBS-K001 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike S1 protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685(Accession # QHD43416.1).

SARS-CoV-2 Spike S2-coupled Magnetic Beads

MBS-K018 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike S2 Protein is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1 (F817P, A892P, A899P, A942P,K986P,V987P)).

SARS-CoV-2 Spike RBD-coupled Magnetic Beads

MBS-K002 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541(Accession # QHD43416.1).

SARS-CoV-2 Spike NTD-coupled Magnetic Beads

MBS-K019 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike NTD Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Phe 318(Accession # QHD43416.1).

Human CD3E & CD3D Heterodimer-coupled Magnetic Beads

MBS-K003 2mg
EUR 2707.1
Description: Human CD3E & CD3D Heterodimer protein is expressed from human 293 cells (HEK293).It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1(CD3E) & P04234-1(CD3D)).

Magnetic Beads-conjugated Rabbit IgG isotype control

AC047 100μL
EUR 87.75
Description: None

SARS-CoV-2 Spike Trimer-coupled Magnetic Beads

MBS-K015 2mg
EUR 4312.1
Description: SARS-CoV-2 Spike Trimer protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (R683A, R685A)).

Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb

AE037 50 ul
EUR 211.2

Magnetic Beads-conjugated Rabbit anti DDDDK-Tag mAb

AE097 1000μL
EUR 84.5

SARS-CoV-2 Spike RBD (B.1.351) Coupled Magnetic Beads

MBS-K032 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD (B.1.351) Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (K417N,E484K,N501Y)).

SARS-CoV-2 Spike RBD (P.1) Coupled Magnetic Beads

MBS-K033 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD (P.1) Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (K417T,E484K,N501Y)).

SARS-CoV-2 Spike RBD (B.1.1.7) Coupled Magnetic Beads

MBS-K034 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike RBD (B.1.1.7) Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1(N501Y)).

resDetect™ resDNA Sample Preparation Kit (Magnetic Beads)

OPA-R005 50preps
EUR 310.3
Description: DNA extraction is a critical step in many molecular biology techniques, such as PCR, sequencing, and cloning, as it is necessary to obtain pure and intact DNA that can be used in downstream applications. DNA extraction kits can simplify and standardize the DNA extraction process, allowing researchers to obtain high-quality DNA from a wide range of samples with reproducible results.

Protein A-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A001 10mg
EUR 866.7
Description: The Protein A-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of protein A. The beads can be used to capture the antibodies in Chemiluminescence procedures.The Protein A is a 42 kDa surface protein, It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family.The Anti-Protein A-coupled Magnetic Beads is easy to capture the antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Protein G-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A002 10mg
EUR 866.7
Description: The Protein G-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of protein G. The beads can be used to capture the antibodies in Chemiluminescence procedures.The Protein G binds the heavy chain within the Fc region of most immunoglobulins but not bind dog immunoglobulins.The Protein G-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

Protein L-coupled Magnetic Beads (recommended for MPCLIA)

MPC-A008 10mg
EUR 866.7
Description: The Protein L-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of Protein L. The beads can be used to capture the antibodies in Chemiluminescence procedures. The Protein L is a 45 kDa surface protein, and can bind almost all antibody types, including IgG, IgM, IgA, IgE and IgD. It binds to antibodies with appropriate Kappa light chains such as VkI, VkIII and VkIV, whereas no binding occurred with antibodies of the VkII or with any Lambda light chains. The Protein L-coupled Magnetic Beads is easy to capture the most antibodies, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.

SARS-CoV-2 (Delta) Spike RBD-coupled Magnetic Beads

MBS-K037 2mg
EUR 2707.1
Description: SARS-CoV-2 (Delta) Spike RBD Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (L452R, T478K)).

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse)

KTI1010-EN-20T 20 T
EUR 139
Description: Antibody coupling magnetic beads, suitable for IP/ CO-IP experiment, protein purification.

SARS-CoV-2 Spike Trimer (D614G)-coupled Magnetic Beads

MBS-K016 2mg
EUR 4312.1
Description: SARS-CoV-2 Spike Trimer (D614G) protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (D614G, R683A, R685A)).

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit)

KTI1020-EN-20T 20 T
EUR 139
Description: Antibody coupling magnetic beads, suitable for IP/ CO-IP experiment, protein purification.

SARS-CoV-2 (Omicron) Spike RBD-coupled Magnetic Beads

MBS-K043 2mg
EUR 2707.1
Description: SARS-CoV-2 (Omicron) Spike RBD Protein is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537(Accession # QHD43416.1 (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H)).

SARS-CoV-2 Spike Trimer (B.1.1.7) Coupled Magnetic Beads

MBS-K029 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike Trimer (B.1.1.7) Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (HV69-70del/Y144del//N501Y/A570D/D614G/P681H/T716I/S982A/D1118H/R683A/R685A/F817P/A892P/A899P/A942P/K986P/V987P)).

SARS-CoV-2 Spike Trimer (B.1.351) Coupled Magnetic Beads

MBS-K030 2mg
EUR 2707.1
Description: SARS-CoV-2 Spike Trimer (B.1.351) Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V, R683A, R685A,F817P, A892P, A899P, A942P, K986P, V987P)).

Chromatin immunoprecipitation (ChIP): revisiting the efficacy of sample preparation, sonication, quantification of sheared DNA, and analysis via PCR.

The “quantitative” ChIP, a tool commonly used to study protein-DNA interactions in cells and tissue, is a difficult assay often plagued with technical error. We present, herein, the process required to merge multiple protocols into a quick, reliable and easy method and an approach to accurately quantify ChIP DNA prior to performing PCR.

 

  • We demonstrate that high intensity sonication for at least 30 min is required for full cellular disruption and maximum DNA recovery because ChIP lysis buffers fail to lyse formaldehyde-fixed cells. In addition, extracting ChIP DNA with chelex-100 yields samples that are too dilute for evaluation of shearing efficiency or quantification via nanospectrophotometry.
  • However, DNA extracted from the Mock-ChIP supernatant via the phenol-chloroform-isoamyl alcohol (PCIA) method can be used to evaluate DNA shearing efficiency and used as the standard in a fluorescence-based microplate assay.
  • This enabled accurate quantification of DNA in chelex-extracted ChIP samples and normalization to total DNA concentration prior to performing real-time PCR (rtPCR). Thus, a quick ChIP assay that can be completed in nine bench hours over two days has been validated along with a rapid, accurate and repeatable way to quantify ChIP DNA.
  • The resulting rtPCR data more accurately depicts treatment effects on protein-DNA interactions of interest.

Rapid quantification methods for genetically modified maize contents using genomic DNAs pretreated by sonication and restriction endonuclease digestion for a capillary-type real-time PCR system with a plasmid reference standard.

For rough quantitative analysis of genetically modified maize contents, rapid methods for measurement of the copy numbers of the cauliflower mosaic virus 35S promoter region (P35S) and MON810 construct-specific gene (MON810) using a combination of a capillary-type real-time PCR system with a plasmid DNA were established.

To reduce the characteristic differences between the plasmid DNA and genomic DNA, we showed that pretreatment of the extracted genomic DNA by a combination of sonication and restriction endonuclease digestion before measurement is effective.

The accuracy and reproducibility of this method for MON810 content (%) at a level of 5.0% MON810 mixed samples were within a range from 4.26 to 5.11% in the P35S copy number quantification. These methods should prove to be a useful tool to roughly quantify GM maize content.

 

Dog Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E08R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E08R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E08R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E07R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E07R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E07R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Microsporum canis

PCR-V600-PCRV60048D PCR-V600-48D
EUR 230

Microsporum canis

PCR-V600-PCRV60096D PCR-V600-96D
EUR 312

Microsporum canis

Oneq-V600-OneqV600100D Oneq-V600-100D
EUR 515

Microsporum canis

Oneq-V600-OneqV600150D Oneq-V600-150D
EUR 594

Microsporum canis

Oneq-V600-OneqV60050D Oneq-V600-50D
EUR 413

Microsporum canis PCR kit

PCR-V600-48D 50T
EUR 543.6

Microsporum canis PCR kit

PCR-V600-96D 100T
EUR 686.4

Microsporum canis RT PCR kit

RTq-V600-100D 100T
EUR 860.4

Microsporum canis RT PCR kit

RTq-V600-150D 150T
EUR 969.6

Microsporum canis RT PCR kit

RTq-V600-50D 50T
EUR 717.6

QPCR Kit DNA Microsporum canis

MOL8800 EACH
EUR 772.92

QPCR Kit DNA Microsporum canis

MOL8802 EACH
EUR 982.68

QPCR Kit DNA Microsporum gypseum

MOL8820 EACH
EUR 772.92

QPCR Kit DNA Microsporum gypseum

MOL8822 EACH
EUR 982.68

Microsporum audouinii PCR kit

PCR-H696-48D 50T
EUR 543.6

Microsporum audouinii PCR kit

PCR-H696-96D 100T
EUR 686.4

Microsporum audouinii RT PCR kit

RTq-H696-100D 100T
EUR 860.4

Microsporum audouinii RT PCR kit

RTq-H696-150D 150T
EUR 969.6

Microsporum audouinii RT PCR kit

RTq-H696-50D 50T
EUR 717.6

Microsporum canis One-Step PCR kit

Oneq-V600-100D 100T
EUR 1039.2

Microsporum canis One-Step PCR kit

Oneq-V600-150D 150T
EUR 1177.2

Microsporum canis One-Step PCR kit

Oneq-V600-50D 50T
EUR 861.6

Microsporum ferrugineum PCR kit

PCR-H697-48D 50T
EUR 543.6

Microsporum ferrugineum PCR kit

PCR-H697-96D 100T
EUR 686.4

Microsporum ferrugineum RT PCR kit

RTq-H697-100D 100T
EUR 860.4

Microsporum ferrugineum RT PCR kit

RTq-H697-150D 150T
EUR 969.6

Microsporum ferrugineum RT PCR kit

RTq-H697-50D 50T
EUR 717.6

Microsporum audouinii One-Step PCR kit

Oneq-H696-100D 100T
EUR 1039.2

Microsporum audouinii One-Step PCR kit

Oneq-H696-150D 150T
EUR 1177.2

Microsporum audouinii One-Step PCR kit

Oneq-H696-50D 50T
EUR 861.6

Microsporum ferrugineum One-Step PCR kit

Oneq-H697-100D 100T
EUR 1039.2

Microsporum ferrugineum One-Step PCR kit

Oneq-H697-150D 150T
EUR 1177.2

Microsporum ferrugineum One-Step PCR kit

Oneq-H697-50D 50T
EUR 861.6

Microspores gypseum PCR kit

PCR-H701-48D 50T
EUR 543.6

Microspores gypseum PCR kit

PCR-H701-96D 100T
EUR 686.4

Microspores gypseum RT PCR kit

RTq-H701-100D 100T
EUR 860.4

Microspores gypseum RT PCR kit

RTq-H701-150D 150T
EUR 969.6

Microspores gypseum RT PCR kit

RTq-H701-50D 50T
EUR 717.6

Microspores gypseum One-Step PCR kit

Oneq-H701-100D 100T
EUR 1039.2

Microspores gypseum One-Step PCR kit

Oneq-H701-150D 150T
EUR 1177.2

Microspores gypseum One-Step PCR kit

Oneq-H701-50D 50T
EUR 861.6

Microspoon 9 In.

43200017-1 1 Each
EUR 5.12

Microspoon 9 In.

43200017-2 5 Each
EUR 18.12

Microspoon 9 In.

43200017-3 10 Each
EUR 42.1

Shallow Microspoon 9 In.

43200018-1 1 Each
EUR 5.23

Shallow Microspoon 9 In.

43200018-2 5 Each
EUR 18.12

Shallow Microspoon 9 In.

43200018-3 10 Each
EUR 43.01
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