Chromatin – DNA fragments by sonication and immunoprecipitated

Detonation Nanodiamond for Monodispersed, Soluble DNA-Nanodiamond Conjugates Using Mixed Silane Bead-Assisted

Zoe
Zoe

Functionalisation of Detonation Nanodiamond for Monodispersed, Soluble DNA-Nanodiamond Conjugates Using Mixed Silane Bead-Assisted Sonication Disintegration.

Nanodiamonds have many attractive properties that make them suitable for a range of biological applications, but their practical use has been limited because nanodiamond conjugates tend to aggregate in solution during or after functionalisation.

 

  • Here we demonstrate the production of DNA-detonation nanodiamond (DNA-DND) conjugates with high dispersion and solubility using an ultrasonic, mixed-silanization chemistry protocol based on the in situ Bead-Assisted Sonication Disintegration (BASD) silanization method.
  • We use two silanes to achieve these properties: (1) 3-(trihydroxysilyl)propyl methylphosphonate (THPMP); a negatively charged silane that imparts high zeta potential and solubility in solution; and (2) (3-aminopropyl)triethoxysilane (APTES); a commonly used functional silane that contributes an amino group for subsequent bioconjugation.
  • We target these amino groups for covalent conjugation to thiolated, single-stranded DNA oligomers using the heterobifunctional crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC).
  • The resulting DNA-DND conjugates are the smallest reported to date, as determined by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). The functionalisation method we describe is versatile and can be used to produce a wide variety of soluble DND-biomolecule conjugates.

Validating sonication as a DNA extraction method for use with carrion flies.

Entomological evidence can be critical in establishing a postmortem interval estimate. DNA-based species identification can be an extremely valuable tool for forensic entomology.

The problem of processing samples in a consistent, cost-effective manner that retains the morphological attributes of the specimen for vouchering has led us to investigate sonication as a primary means of non-destructive DNA extraction from carrion flies.

We analyze the efficacy of this technique and compare it to an established DNA extraction technique – the Qiagen DNeasy tissue kit. Our results indicate that sonication produces a significant reduction in the sequence length and lower PHRED quality scores when compared to sequences using DNA obtained using the DNeasy kit, but species identification and phylogenetic inferences between sonication and DNeasy extractions are equivalent.

chipready
chipready

Recombinant Listeria Monocytogenes Serotype 4a rnc Protein (aa 1-229)

VAng-Ly0179-50gEcoli 50 µg (E. coli)
EUR 2138.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribonuclease 3, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rnz Protein (aa 1-306)

VAng-Ly0182-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribonuclease Z, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rph Protein (aa 1-248)

VAng-Ly0183-50gEcoli 50 µg (E. coli)
EUR 2208
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribonuclease PH, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a tig Protein (aa 1-427)

VAng-Ly0240-50gEcoli 50 µg (E. coli)
EUR 2853.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Trigger factor, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a tmk Protein (aa 1-208)

VAng-Ly0241-50gEcoli 50 µg (E. coli)
EUR 2053.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Thymidylate kinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a tsf Protein (aa 1-294)

VAng-Ly0249-50gEcoli 50 µg (E. coli)
EUR 2377.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Elongation factor Ts, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a udk Protein (aa 1-209)

VAng-Ly0252-50gEcoli 50 µg (E. coli)
EUR 2067.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Uridine kinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a upp Protein (aa 1-209)

VAng-Ly0253-50gEcoli 50 µg (E. coli)
EUR 2067.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Uracil phosphoribosyltransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b ade Protein (aa 1-579)

VAng-Ly0264-inquire inquire Ask for price
Description: Listeria Monocytogenes serotype 4b (strain F2365) Adenine deaminase, partial, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b ddl Protein (aa 1-370)

VAng-Ly0319-50gEcoli 50 µg (E. coli)
EUR 2629.2
Description: Listeria Monocytogenes serotype 4b (strain F2365) D-alanine--D-alanine ligase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b gmk Protein (aa 1-205)

VAng-Ly0356-50gEcoli 50 µg (E. coli)
EUR 2040
Description: Listeria Monocytogenes serotype 4b (strain F2365) Guanylate kinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b stp Protein (aa 1-252)

VAng-Ly0702-50gEcoli 50 µg (E. coli)
EUR 2222.4
Description: Listeria Monocytogenes serotype 4b (strain F2365) Serine/threonine phosphatase stp, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b tdk Protein (aa 1-191)

VAng-Ly0706-50gEcoli 50 µg (E. coli)
EUR 2011.2
Description: Listeria Monocytogenes serotype 4b (strain F2365) Thymidine kinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a tuf Protein (aa 1-395)

VAng-Ly0788-50gEcoli 50 µg (E. coli)
EUR 2727.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Elongation factor Tu, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b eno Protein (aa 1-430)

VAng-Ly0812-50gEcoli 50 µg (E. coli)
EUR 2853.6
Description: Listeria Monocytogenes serotype 4b (strain F2365) Enolase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b fni Protein (aa 1-358)

VAng-Ly0815-50gEcoli 50 µg (E. coli)
EUR 2601.6
Description: Listeria Monocytogenes serotype 4b (strain Clip81459) Isopentenyl-diphosphate delta-isomerase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b obg Protein (aa 1-429)

VAng-Ly0878-50gEcoli 50 µg (E. coli)
EUR 2853.6
Description: Listeria Monocytogenes serotype 4b (strain F2365) GTPase obg, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b rny Protein (aa 1-520)

VAng-Ly0901-inquire inquire Ask for price
Description: Listeria Monocytogenes serotype 4b (strain F2365) Ribonuclease Y, partial, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a adk Protein (aa 1-215)

VAng-Ly0010-50gEcoli 50 µg (E. coli)
EUR 2096.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Adenylate kinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a alr Protein (aa 1-368)

VAng-Ly0011-50gEcoli 50 µg (E. coli)
EUR 2629.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Alanine racemase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a apt Protein (aa 1-173)

VAng-Ly0012-50gEcoli 50 µg (E. coli)
EUR 1956
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Adenine phosphoribosyltransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a cca Protein (aa 1-393)

VAng-Ly0021-50gEcoli 50 µg (E. coli)
EUR 2713.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) CCA-adding enzyme, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a cmk Protein (aa 1-224)

VAng-Ly0025-50gEcoli 50 µg (E. coli)
EUR 2124
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Cytidylate kinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a def Protein (aa 1-183)

VAng-Ly0035-50gEcoli 50 µg (E. coli)
EUR 1969.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Peptide deformylase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a der Protein (aa 1-436)

VAng-Ly0038-50gEcoli 50 µg (E. coli)
EUR 3072
Description: Listeria Monocytogenes serotype 4a (strain HCC23) GTPase Der, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a efp Protein (aa 1-185)

VAng-Ly0043-50gEcoli 50 µg (E. coli)
EUR 1969.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Elongation factor P, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a era Protein (aa 1-301)

VAng-Ly0045-50gEcoli 50 µg (E. coli)
EUR 2390.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) GTPase Era, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a fhs Protein (aa 1-560)

VAng-Ly0047-inquire inquire Ask for price
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Formate--tetrahydrofolate ligase, partial, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a fmt Protein (aa 1-312)

VAng-Ly0048-50gEcoli 50 µg (E. coli)
EUR 2432.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Methionyl-tRNA formyltransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a frr Protein (aa 1-185)

VAng-Ly0051-50gEcoli 50 µg (E. coli)
EUR 1969.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribosome-recycling factor, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a serS Protein (aa 1-427)

VAng-Ly0234-50gEcoli 50 µg (E. coli)
EUR 2853.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Serine--tRNA ligase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4b serC Protein (aa 1-363)

VAng-Ly0695-50gEcoli 50 µg (E. coli)
EUR 2614.8
Description: Listeria Monocytogenes serotype 4b (strain F2365) Phosphoserine aminotransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a luxS Protein (aa 1-155)

VAng-Ly0129-50gEcoli 50 µg (E. coli)
EUR 1885.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) S-ribosylhomocysteine lyase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a metK Protein (aa 1-399)

VAng-Ly0130-50gEcoli 50 µg (E. coli)
EUR 2727.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) S-adenosylmethionine synthase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a mgsA Protein (aa 1-134)

VAng-Ly0131-50gEcoli 50 µg (E. coli)
EUR 1815.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Methylglyoxal synthase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a minC Protein (aa 1-225)

VAng-Ly0132-50gEcoli 50 µg (E. coli)
EUR 2124
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Probable septum site-determining protein MinC, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a mntR Protein (aa 1-142)

VAng-Ly0133-50gEcoli 50 µg (E. coli)
EUR 1843.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Transcriptional regulator MntR, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a mraZ Protein (aa 1-143)

VAng-Ly0134-50gEcoli 50 µg (E. coli)
EUR 1843.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Protein MraZ, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a murB Protein (aa 1-298)

VAng-Ly0136-50gEcoli 50 µg (E. coli)
EUR 2390.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) UDP-N-acetylenolpyruvoylglucosamine reductase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a murI Protein (aa 1-266)

VAng-Ly0137-50gEcoli 50 µg (E. coli)
EUR 2264.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Glutamate racemase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a mutL Protein (aa 1-603)

VAng-Ly0138-inquire inquire Ask for price
Description: Listeria Monocytogenes serotype 4a (strain HCC23) DNA mismatch repair protein mutL, partial, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a mutS Protein (aa 1-860)

VAng-Ly0139-inquire inquire Ask for price
Description: Listeria Monocytogenes serotype 4a (strain HCC23) DNA mismatch repair protein MutS, partial, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a nadA Protein (aa 1-366)

VAng-Ly0141-50gEcoli 50 µg (E. coli)
EUR 2614.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Quinolinate synthase A, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a nadE Protein (aa 1-274)

VAng-Ly0142-50gEcoli 50 µg (E. coli)
EUR 2292
Description: Listeria Monocytogenes serotype 4a (strain HCC23) NH (3)-dependent NAD (+) synthetase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a nagB Protein (aa 1-234)

VAng-Ly0143-50gEcoli 50 µg (E. coli)
EUR 2151.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Glucosamine-6-phosphate deaminase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a namA Protein (aa 1-338)

VAng-Ly0144-50gEcoli 50 µg (E. coli)
EUR 2517.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) NADPH dehydrogenase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a nrdR Protein (aa 1-266)

VAng-Ly0147-50gEcoli 50 µg (E. coli)
EUR 1870.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Transcriptional repressor NrdR, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a panD Protein (aa 1-24)

VAng-Ly0149-50gEcoli 50 µg (E. coli)
EUR 1436.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Aspartate 1-decarboxylase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a pepT Protein (aa 1-410)

VAng-Ly0150-50gEcoli 50 µg (E. coli)
EUR 2784
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Peptidase T, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a pfkA Protein (aa 1-319)

VAng-Ly0151-50gEcoli 50 µg (E. coli)
EUR 2446.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 6-phosphofructokinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a plsX Protein (aa 1-337)

VAng-Ly0153-50gEcoli 50 µg (E. coli)
EUR 2517.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Phosphate acyltransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a plsY Protein (aa 1-198)

VAng-Ly0154-inquire inquire Ask for price
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Glycerol-3-phosphate acyltransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a ppaX Protein (aa 1-217)

VAng-Ly0156-50gEcoli 50 µg (E. coli)
EUR 2096.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Pyrophosphatase ppaX, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a prfA Protein (aa 1-358)

VAng-Ly0157-50gEcoli 50 µg (E. coli)
EUR 2601.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Peptide chain release factor 1, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a prmA Protein (aa 1-314)

VAng-Ly0158-50gEcoli 50 µg (E. coli)
EUR 2432.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribosomal protein L11 methyltransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a proB Protein (aa 1-276)

VAng-Ly0159-50gEcoli 50 µg (E. coli)
EUR 2306.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Glutamate 5-kinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a proS Protein (aa 1-568)

VAng-Ly0160-1mgEcoli 1 mg (E. coli)
EUR 7388.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Proline--tRNA ligase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a purC Protein (aa 1-237)

VAng-Ly0163-50gEcoli 50 µg (E. coli)
EUR 2151.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Phosphoribosylaminoimidazole-succinocarboxamide synthase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a purM Protein (aa 1-349)

VAng-Ly0164-50gEcoli 50 µg (E. coli)
EUR 2572.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Phosphoribosylformylglycinamidine cyclo-ligase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a pyrB Protein (aa 1-303)

VAng-Ly0165-50gEcoli 50 µg (E. coli)
EUR 2404.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Aspartate carbamoyltransferase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a pyrC Protein (aa 1-426)

VAng-Ly0166-50gEcoli 50 µg (E. coli)
EUR 2826
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Dihydroorotase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rbfA Protein (aa 1-114)

VAng-Ly0167-50gEcoli 50 µg (E. coli)
EUR 1744.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribosome-binding factor A, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a recA Protein (aa 1-348)

VAng-Ly0168-50gEcoli 50 µg (E. coli)
EUR 2572.8
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Protein RecA, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a recF Protein (aa 1-370)

VAng-Ly0169-50gEcoli 50 µg (E. coli)
EUR 2629.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) DNA replication and repair protein recF, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a recO Protein (aa 1-255)

VAng-Ly0170-50gEcoli 50 µg (E. coli)
EUR 2222.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) DNA repair protein recO, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a recR Protein (aa 1-198)

VAng-Ly0171-50gEcoli 50 µg (E. coli)
EUR 2025.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Recombination protein RecR, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a recU Protein (aa 1-201)

VAng-Ly0172-50gEcoli 50 µg (E. coli)
EUR 2040
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Holliday junction resolvase recU, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a recX Protein (aa 1-269)

VAng-Ly0173-50gEcoli 50 µg (E. coli)
EUR 2264.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Regulatory protein recX, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rhaA Protein (aa 1-420)

VAng-Ly0174-50gEcoli 50 µg (E. coli)
EUR 2811.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) L-rhamnose isomerase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rhaB Protein (aa 1-483)

VAng-Ly0175-50gEcoli 50 µg (E. coli)
EUR 3050.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Rhamnulokinase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rhaD Protein (aa 1-273)

VAng-Ly0176-50gEcoli 50 µg (E. coli)
EUR 2292
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Rhamnulose-1-phosphate aldolase, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rimM Protein (aa 1-172)

VAng-Ly0177-50gEcoli 50 µg (E. coli)
EUR 1941.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribosome maturation factor RimM, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rimP Protein (aa 1-155)

VAng-Ly0178-50gEcoli 50 µg (E. coli)
EUR 1885.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribosome maturation factor RimP, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rnhB Protein (aa 1-261)

VAng-Ly0180-50gEcoli 50 µg (E. coli)
EUR 2250
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribonuclease HII, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rnpA Protein (aa 1-119)

VAng-Ly0181-50gEcoli 50 µg (E. coli)
EUR 1759.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) Ribonuclease P protein component, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplA Protein (aa 1-229)

VAng-Ly0184-50gEcoli 50 µg (E. coli)
EUR 2138.4
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L1, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplC Protein (aa 1-209)

VAng-Ly0185-50gEcoli 50 µg (E. coli)
EUR 2067.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L3, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplD Protein (aa 1-207)

VAng-Ly0186-50gEcoli 50 µg (E. coli)
EUR 2053.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L4, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplE Protein (aa 1-179)

VAng-Ly0187-50gEcoli 50 µg (E. coli)
EUR 1969.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L5, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplF Protein (aa 1-178)

VAng-Ly0188-50gEcoli 50 µg (E. coli)
EUR 1956
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L6, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplI Protein (aa 1-148)

VAng-Ly0189-50gEcoli 50 µg (E. coli)
EUR 1857.6
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L9, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplJ Protein (aa 1-166)

VAng-Ly0190-50gEcoli 50 µg (E. coli)
EUR 1927.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L10, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplK Protein (aa 1-141)

VAng-Ly0191-50gEcoli 50 µg (E. coli)
EUR 1843.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L11, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplL Protein (aa 1-120)

VAng-Ly0192-50gEcoli 50 µg (E. coli)
EUR 1759.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L7/L12, recombinant protein.

Recombinant Listeria Monocytogenes Serotype 4a rplM Protein (aa 1-145)

VAng-Ly0193-50gEcoli 50 µg (E. coli)
EUR 1843.2
Description: Listeria Monocytogenes serotype 4a (strain HCC23) 50S ribosomal protein L13, recombinant protein.

Physisorption of DNA molecules on chemically modified single-walled carbon nanotubes with and without sonication.

We investigated the physisorption phenomenon of single-stranded DNA (ssDNA) molecules onto two types of commercially available chemically functionalized single-walled carbon nanotubes (SWNTs) by atomic force microscopy (AFM) and agarose gel electrophoresis.

We found that DNA molecules can adsorb on the water-soluble SWNT surfaces without sonication, although sonication treatment has been used for hybridization of DNA and SWNTs in many previous studies.

Using our method, damage of DNA molecules by sonication can be avoided. On the other hand, the amount of DNA molecules adsorbed on SWNT surfaces increased when the samples were sonicated.

This fact suggests that the sonication is effective not only at debundling of SWNTs, but also at assisting DNA adsorption. Furthermore, DNA adsorption was affected by the types of functionalized SWNTs.

In the case of SWNTs functionalized with polyethylene glycol (PEG-SWNT), physisorption of ssDNA molecules was confirmed only by agarose-gel electrophoresis. In contrast, amino-terminated SWNTs (NH2-SWNTs) showed a change in the height distribution profile based on AFM observations.

These results suggest that DNA molecules tended to adsorb to NH2-SWNT surfaces, although DNA molecules can also adsorb on PEG-SWNT surfaces. Our results revealed fundamental information for developing nanobiodevices using hybrids of DNA and SWNTs.

G-quadruplex DNA-based asymmetric catalysis of michael addition: Effects of sonication, ligands, and co-solvents.

There is an escalating interest of using double stranded DNA molecules as a chiral scaffold to construct metal-biomacromolecule hybrid catalysts for asymmetric synthesis. Several recent studies also evaluated the use of G-quadruplex DNA-based catalysts for asymmetric Diels-Alder and Friedel-Crafts reactions.

 

  • However, there is still a lack of understanding of how different oligonucleotides, salts (such as NaCl and KCl), metal ligands and co-solvents affect the catalytic performance of quadruplex DNA-based hybrid catalysts. In this study, we aim to systematically evaluate these key factors in asymmetric Michael addition reactions, and to examine the conformational and molecular changes of DNA by circular dichroism (CD) spectroscopy and gel electrophoresis.
  • We achieved up to 95% yield and 50% enantiomeric excess (ee) when the reaction of 2-acylimidazole 1a and dimethylmalonate was catalyzed by 5′-G3 (TTAG3 )3 -3′ (G4DNA1) in 20 mM MOPS (pH 6.5) containing 50 mM KCl and 40 µM [Cu(dmbipy)(NO3 )2 ], and G4DNA1 was pre-sonicated in ice bath for 10 min prior to the reaction. G-quadruplex-based hybrid catalysts provide a new tool for asymmetric catalysis, but future mechanistic studies should be sought to further improve the catalytic efficiency.
  • The current work presents a systematic study of asymmetric Michael addition catalyzed by G-quadruplex catalysts constructed via non-covalent complexing, and an intriguing finding of the effect of pre-sonication on catalytic efficiency. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:891-898, 2016.

 

PE anti-mouse CD226 (DNAM-1)

E16FMP226-025U 25 μg
EUR 433.33
Description: Available in various conjugation types.

PE anti-mouse CD226 (DNAM-1)

E16FMP226-100U 100 μg
EUR 1148.33
Description: Available in various conjugation types.

APC anti-mouse CD226 (DNAM-1)

E16FMA226-025U 25 μg
EUR 498.33
Description: Available in various conjugation types.

APC anti-mouse CD226 (DNAM-1)

E16FMA226-100U 100 μg
EUR 1256.67
Description: Available in various conjugation types.

FITC anti-mouse CD226 (DNAM-1)

E16FMF226-050U 50 μg
EUR 541.67
Description: Available in various conjugation types.

PE Rabbit anti-Human DNAM-1/CD226 mAb

A23591 25μg
EUR 1557.4
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 250-473 of human MEF2A+MEF2C (Q06413).

Mouse Monoclonal anti-Human CD226 (DNAM-1) Antibody

xAP-0182 100ug
EUR 280

Mouse Monoclonal anti-Human CD226 (DNAM-1) Antibody

xAP-0183 100ug
EUR 280

Human DNAM-1 / CD226 Protein, Fc Tag

DN1-H5257 1mg
EUR 267.5
Description: Human DNAM-1, Fc Tag (DN1-H5257) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).

Human DNAM-1 / CD226 Protein, His Tag

DN1-H52H6 1mg
EUR 267.5
Description: Human DNAM-1, His Tag (DN1-H52H6) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).

Human DNAM-1 Protein, mFc-His Tag

E33P100050 10 μg
EUR 225

CD226/DNAM-1 Antibody

E18-0087-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

CD226/DNAM-1 Antibody

E18-0087-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

CD226/DNAM-1 Antibody

E11-184778 100ug/100ul
EUR 225
Description: Available in various conjugation types.

CD226/DNAM-1 Antibody

AF0087 200ul
EUR 420

CD226/DNAM-1 Antibody

AF4778 200ul
EUR 540

CD226/DNAM-1 Antibody

AF4778-100ul 100ul
EUR 350

CD226/DNAM-1 Antibody

AF4778-200ul 200ul
EUR 450

CD226/DNAM-1 Antibody

AF4778-50ul 50ul
EUR 250

CD226 / DNAM-1 Antibody

20-abx119383
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  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Cd226 Antibody / Dnam-1

RQ6373 100 ug
EUR 356.15
Description: DNAM 1, also known as CD226, is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes. This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.

CD226 Antibody / DNAM-1

RQ6841 100ug
EUR 356.15
Description: DNAM 1, also known as CD226, is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes. This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.

NE Purified anti-mouse CD226 (DNAM-1)

E16FMY226-500U 500 μg
EUR 1625
Description: Available in various conjugation types.

DNAM-1 Polyclonal Antibody

41787-50ul 50ul
EUR 224.4

DNAM-1 Polyclonal Antibody

41787-100ul 100ul
EUR 302.4

DNAM-1 Polyclonal Antibody

40846-100ul 100ul
EUR 302.4

DNAM-1 Polyclonal Antibody

40846-50ul 50ul
EUR 224.4

DNAM-1 Polyclonal Antibody

E040846 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNAM-1 Polyclonal Antibody

E041787 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNAM-1 Polyclonal Antibody

E20-71381 100ug
EUR 225
Description: Available in various conjugation types.

DNAM-1 Polyclonal Antibody

E20-75402 100ug
EUR 225
Description: Available in various conjugation types.

DNAM-1 Polyclonal Antibody

ABP53133-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53133-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53133-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of DNAM-1 from Human. This DNAM-1 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human DNAM-1

DNAM-1 Polyclonal Antibody

ABP53533-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

ABP53533-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

ABP53533-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

DNAM-1 Polyclonal Antibody

E44H06844 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

DNAM-1 Polyclonal Antibody

E44H01772 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Rabbit Polyclonal Anti-CD226/DNAM-1 Antibody

TA347323 100 µg Ask for price

OKRC00369-96W - DNAM-1 ELISA Kit (Human)

OKRC00369-96W 96Wells
EUR 525

Active Recombinant Human DNAM-1/CD226 Protein

RP00283 10 μg
EUR 78

dNAM

D100545 500mg
EUR 4500
Description: 1117893-19-2

CD226/DNAM- 1 Antibody

ABF0087 100 ug
EUR 525.6

Human DNAM-1/CD226 ELISA Kit PicoKine®

EK2161 96 wells/kit, with removable strips.
EUR 499
Description: Human DNAM-1/CD226 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD226 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

AP76445 1mg
EUR 1879

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-10ug 10ug
EUR 151.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-1mg 1mg
EUR 1581.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-500ug 500ug
EUR 1155.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-50ug 50ug
EUR 333.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human DNAM-1 Protein, Avi & His Tag

E40KMP1218 20ug
EUR 495

Recombinant Human DNAM-1 Protein, Avi & His Tag

E40KMP1892 20ug
EUR 495

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

AP76444 1mg
EUR 1879

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-10ug 10ug
EUR 151.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-1mg 1mg
EUR 1581.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-500ug 500ug
EUR 1155.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-50ug 50ug
EUR 333.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

ABflo® 488 Rabbit anti-Human DNAM-1/CD226 mAb

A23175 25μg
EUR 213.2
Description: Recombinant protein of human CD16.

ABflo® 647 Rabbit anti-Human DNAM-1/CD226 mAb

A23176 25μg
EUR 213.2
Description: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CES1 (P23141).

Rat Monoclonal anti-Mouse CD226 (DNAM-1) Antibody

xAP-0583 100ug
EUR 280

Rat Monoclonal anti-Mouse CD226 (DNAM-1) Antibody

xAP-0584 100ug
EUR 280

DNAM-1 Rabbit pAb

E2252268 100ul
EUR 225
Description: Available in various conjugation types.

DNAM-1 Rabbit Polyclonal Antibody

ES4132-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Rabbit Polyclonal Antibody

ES4132-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Rabbit Polyclonal Antibody

ES4532-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 Rabbit Polyclonal Antibody

ES4532-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Recombinant Human CD226/DNAM-1 Protein (His Tag)

PKSH033728-10ug 10ug
EUR 92
Description: Human

Recombinant Human CD226/DNAM-1 Protein (His Tag)

PKSH033728-50ug 50ug
EUR 268
Description: Human

DNAM-1 Recombinant Protein

96-863 0.1 mg
EUR 588.3
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

DNAM-1 Recombinant Protein

96-864 0.1 mg
EUR 588.3
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

DNAM-1 Polyclonal Conjugated Antibody

C40846 100ul
EUR 476.4

Alexa Fluor® 647 anti-mouse CD226 (DNAM-1)

E16FMK226-100U 100 μg
EUR 1278.33
Description: Available in various conjugation types.

CD226/DNAM-1 (Ab-329) Antibody

E11-0843B 100μg
EUR 225
Description: Available in various conjugation types.

CD226/DNAM-1 (Ab-329) Antibody

8B0843 50ug
EUR 368
Description: CD226/DNAM-1 (Ab-329) Antibody

Biotinylated Human DNAM-1 / CD226 Protein, Fc,Avitag™

DN1-H82F9 25ug
EUR 1722.7
Description: Biotinylated Human DNAM-1, Fc,Avitag (DN1-H82F9) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).

Human CD226/DNAM-1 protein, His tag (Animal-Free)

PRP2038-100ug 100 μg
EUR 259
Description: Human CD226/DNAM-1 protein, His tag (Animal-Free), expressed in HEK 293 Cells

Human CD226/DNAM-1 protein, His tag (Animal-Free)

PRP2038-10ug 10 μg
EUR 59
Description: Human CD226/DNAM-1 protein, His tag (Animal-Free), expressed in HEK 293 Cells

Human CD226/DNAM-1 protein, His tag (Animal-Free)

PRP2038-1mg 1 mg
EUR 2369
Description: Human CD226/DNAM-1 protein, His tag (Animal-Free), expressed in HEK 293 Cells

Human CD226/DNAM-1 protein, His tag (Animal-Free)

PRP2038-50ug 50 μg
EUR 139
Description: Human CD226/DNAM-1 protein, His tag (Animal-Free), expressed in HEK 293 Cells

Human DNAM-1 / CD226 Recombinant Protein His Tag Lyophilized

IHUDNAM1CD226RHISLY100UG each
EUR 851
Description: Human DNAM-1 / CD226 Recombinant Protein His Tag Lyophilized

CD226/DNAM-1 Rabbit mAb

A23200 20μL
EUR 213.2
Description: Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of mouse UCP1+UCP3 (P12242).

Recombinant Human CD226/DNAM-1 Protein (Fc Tag)(Active)

PKSH033729-10ug 10ug
EUR 92
Description: Human

Recombinant Human CD226/DNAM-1 Protein (Fc Tag)(Active)

PKSH033729-50ug 50ug
EUR 268
Description: Human

Recombinant Human CD226/DNAM-1 Protein (His Tag)(Active)

PKSH031523-100ug 100ug
EUR 732
Description: Human

CD226/DNAM-1 (Phospho-Ser329) Antibody

E11-0843A 100μg
EUR 225
Description: Available in various conjugation types.

CD226/DNAM-1 (Phospho-Ser329) Antibody

E11-184478 100ug/100ul
EUR 225
Description: Available in various conjugation types.
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