Chromatin – DNA fragments by sonication and immunoprecipitated
chipready

Nanogram-Range Immunoprecipitated DNA in ChIP-seq Application

Zoe
Zoe

Purification of nanogram-range immunoprecipitated DNA in ChIP-seq utility

Chromatin immunoprecipitation-sequencing (ChIP-seq) is a broadly used epigenetic strategy for investigating genome-wide protein-DNA interactions in cells and tissues. The strategy has been comparatively properly established however a number of key steps nonetheless require additional enchancment.
As part of the process, immnoprecipitated DNA should endure purification and library preparation for subsequent high-throughput sequencing. Present ChIP protocols sometimes yield nanogram portions of immunoprecipitated DNA primarily relying on the goal of curiosity and beginning chromatin enter quantity. Nonetheless, little info exists on the efficiency of reagents used for the purification of such minute quantities of immunoprecipitated DNA in ChIP elution buffer and their results on ChIP-seq knowledge.
Right here, we in contrast DNA restoration, library preparation effectivity, and ChIP-seq outcomes obtained with a number of business DNA purification reagents utilized to 1 ng ChIP DNA and in addition investigated the impression of situations underneath which ChIP DNA is saved.
We in contrast DNA restoration of ten business DNA purification reagents and phenol/chloroform extraction from 1 to 50 ng of immunopreciptated DNA in ChIP elution buffer. The restoration yield was considerably totally different with 1 ng of DNA whereas comparable in greater DNA quantities.
We additionally noticed that the low nanogram vary of purified DNA is vulnerable to loss throughout storage relying on the kind of polypropylene tube used. The immunoprecipitated DNA equal to 1 ng of purified DNA was topic to DNA purification and library preparation to guage the efficiency of 4 higher performing purification reagents in ChIP-seq functions.
Quantification of library DNAs indicated the chosen purification kits have a negligible impression on the effectivity of library preparation. The ensuing ChIP-seq knowledge have been comparable with the dataset generated by ENCODE consortium and have been extremely correlated between the info from totally different purification reagents.
This examine offers comparative knowledge on business DNA purification reagents utilized to nanogram-range immunopreciptated ChIP DNA and proof for the significance of storage situations of low nanogram-range purified DNA. We verified constant excessive efficiency of a subset of the examined reagents. These outcomes will facilitate the development of ChIP-seq methodology for low-input functions.
chipready
chipready

DNAM-1 Polyclonal Antibody

ABP53533-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of DNAM-1 from Human, Mouse, Monkey. This DNAM-1 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329

CD226/DNAM-1 Antibody

AF4778 200ul
EUR 540

CD226/DNAM-1 Antibody

AF0087 200ul
EUR 420

DNAM-1 Polyclonal Antibody

ES4132-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4132-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 Polyclonal Antibody

ES4532-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against DNAM-1 from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 Polyclonal Antibody

41787-100ul 100ul
EUR 302.4

DNAM-1 Polyclonal Antibody

41787-50ul 50ul
EUR 224.4

DNAM-1 Polyclonal Antibody

40846-100ul 100ul
EUR 302.4

DNAM-1 Polyclonal Antibody

40846-50ul 50ul
EUR 224.4

CD226 / DNAM-1 Antibody

20-abx119383
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

CD226/DNAM- 1 Antibody

ABF0087 100 ug
EUR 525.6

DNAM-1 Recombinant Protein

96-863 0.1 mg
EUR 588.3
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

DNAM-1 Recombinant Protein

96-864 0.1 mg
EUR 588.3
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Cd226 Antibody / Dnam-1

RQ6373 100 ug
EUR 419
Description: DNAM 1, also known as CD226, is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes. This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.

CD226 Antibody / DNAM-1

RQ6841 100ug
EUR 419
Description: DNAM 1, also known as CD226, is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes. This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

CD226/DNAM-1 Blocking Peptide

AF4778-BP 1mg
EUR 234

CD226/DNAM-1 Blocking Peptide

AF0087-BP 1mg
EUR 234

DNAM-1 Polyclonal Conjugated Antibody

C40846 100ul
EUR 476.4

DNAM-1 / CD226 Recombinant Protein

11-431 0.1 mg
EUR 714.3
Description: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

DNAM-1 (phospho Ser329) Polyclonal Antibody

ABP53532-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of DNAM-1 phospho Ser329) from Human, Mouse, Monkey. This DNAM-1 phospho Ser329) antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human DNAM-1 around the phosphorylation site of S329

Phospho-CD226/DNAM-1 (Ser329) Antibody

AF4478 200ul
EUR 540

DNAM-1 (phospho Ser329) Polyclonal Antibody

ES4531-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against DNAM-1 (phospho Ser329) from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 (phospho Ser329) Polyclonal Antibody

ES4531-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against DNAM-1 (phospho Ser329) from Human/Mouse/Monkey. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

DNAM-1 (Phospho-Ser329) Polyclonal Antibody

12361-100ul 100ul
EUR 302.4

DNAM-1 (Phospho-Ser329) Polyclonal Antibody

12361-50ul 50ul
EUR 224.4

HRP-Goat Anti-Mouse Secondary Antibody

A12003
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

HRP-Goat Anti-Rabbit Secondary Antibody

A12004
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

Goat Anti-Rabbit Secondary Antibody, Biotin Conjugated

A12001
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

Goat Anti-Rat Secondary Antibody, Biotin Conjugated

A12002
  • EUR 232.26
  • EUR 117.70
  • 350 µg
  • 350 ug

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

Human DNA methyltransferase,DNAM ELISA Kit

201-12-1975 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human DNA methyltransferase(DNAM)ELISA Kit

GA-E1991HM-48T 48T
EUR 346.8

Human DNA methyltransferase(DNAM)ELISA Kit

GA-E1991HM-96T 96T
EUR 559.2

Human DNA methyltransferase(DNAM)ELISA Kit

QY-E04972 96T
EUR 433.2

Phospho-CD226/DNAM-1 (Ser329) Blocking Peptide

AF4478-BP 1mg
EUR 234

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-10ug 10ug
EUR 151.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-1mg 1mg
EUR 1581.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-500ug 500ug
EUR 1155.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-6His)

CS89-50ug 50ug
EUR 333.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-10ug 10ug
EUR 151.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-1mg 1mg
EUR 1581.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-500ug 500ug
EUR 1155.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Human CD226 Antigen/DNAM-1/CD226 (C-Fc)

CS90-50ug 50ug
EUR 333.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Human Genomic DNA 

X11000
  • Ask for price
  • EUR 235.40
  • 0.2 ml
  • 0.2 ml

Human Brain Genomic DNA  

X11001
  • Ask for price
  • EUR 77.00
  • 10 µg
  • 10 ul

DNAM-1 (Phospho-Ser329) Polyclonal Polyclonal Conjugated Antibody

C12361 100ul
EUR 476.4

Recombinant DNAM-1 Protein (Glu 19-Phe 252)

VAng-1466Lsx-100g 100 µg
EUR 1215.6
Description: Cynomolgus DNAM-1 is expressed in HEK 293 cells. (Uniprot ID: A0A2K5W1Z6-1)

Recombinant DNAM-1 Protein (Glu 19-Phe 252)

VAng-1466Lsx-1mg 1 mg
EUR 7682.4
Description: Cynomolgus DNAM-1 is expressed in HEK 293 cells. (Uniprot ID: A0A2K5W1Z6-1)

Rat DNA methyltransferase(DNAM)ELISA Kit

GA-E0805RT-48T 48T
EUR 380.4

Rat DNA methyltransferase(DNAM)ELISA Kit

GA-E0805RT-96T 96T
EUR 595.2

Rat DNA methyltransferase(DNAM)ELISA Kit

QY-E10847 96T
EUR 433.2

Mouse DNA methyltransferase(DNAM)ELISA Kit

QY-E20479 96T
EUR 433.2

Anti-Apaf-1 (human) Monoclonal Antibody (2E12)

M00889-1 100ug
EUR 518.4
Description: Rat Monoclonal Apaf-1 (human) Antibody (2E12). Validated in ELISA, IP, IF, WB and tested in Human.

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc]

VAng-1463Lsx-100g 100 µg
EUR 885.6
Description: Human DNAM-1, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc]

VAng-1463Lsx-1mg 1 mg
EUR 5008.8
Description: Human DNAM-1, Fc tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [His]

VAng-1465Lsx-100g 100 µg
EUR 885.6
Description: Human DNAM-1, His tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [His]

VAng-1465Lsx-1mg 1 mg
EUR 5008.8
Description: Human DNAM-1, His tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-10ug 10ug
EUR 175.2
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse DNAX Accessory Molecule-1/DNAM-1/CD226 (C-6His)

CS07-50ug 50ug
EUR 363.6
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Anti-Periphilin 1 Antibody

A06996-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Periphilin 1 Antibody (PPHLN1) detection.tested for WB in Human, Mouse.

Anti-Lyl-1 Antibody

A07491-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Lyl-1 Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-GLI-1 Antibody

A07972-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat.

Anti-Cerebellin 1 Antibody

A09176-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat.

Anti-DOC-1 Antibody

A09467-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse.

Anti-NPDC-1 Antibody

A12846-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat.

Anti-ROBO-1 Antibody

A01530-1 100ug
EUR 546
Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-Bag-1 Antibody

A02423-1 50 ul
EUR 476.4
Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat.

Anti-TUB 1 Antibody

A02917-1 100ug/vial
EUR 352.8

Anti-Dok-1 Antibody

A03039-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat.

Anti-TFIIIB90-1 Antibody

A03761-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse.

Anti-Atrophin-1 Antibody

A03828-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat.

Anti-CNG-1 Antibody

A05494-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat.

Anti-PAI-1 Antibody

A00637-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat.

Anti-Flk-1 Antibody

A00901-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse.

Anti-EDG-1 Antibody

A01502-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat.

Anti-PARP-1 Antibody

A00122-1 100ul
EUR 476.4
Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse.

Anti-Presenilin 1 Antibody

A00138-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat.

Anti-P-glycoprotein (human) Monoclonal Antibody (JSB-1)

M00049-1 125ug
EUR 703.2
Description: Mouse Monoclonal P-glycoprotein (human) Antibody (JSB-1). Validated in IF and tested in Human.

SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag

E80025
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc] [Avi]

VAng-1464Lsx-200g 200 µg
EUR 4612.8
Description: Biotinylated Human DNAM-1, Fc tag and Avi tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Recombinant DNAM-1 Protein (Glu 19-Asn 247) [Fc] [Avi]

VAng-1464Lsx-25g 25 µg
EUR 1215.6
Description: Biotinylated Human DNAM-1, Fc tag and Avi tag, is expressed in HEK 293 cells. (Uniprot ID: NP_006557)

Anti-HLA-G (human) Monoclonal Antibody (MEM-G/1)

M01235-1 100ug
EUR 476.4
Description: Mouse Monoclonal HLA-G (human) Antibody (MEM-G/1). Validated in IHC, WB and tested in Human.

Anti-Pyrophosphatase 1/PPA1 Antibody

A07485-1 100ug/vial
EUR 400.8

Microarray evaluation of chromatin-immunoprecipitated DNA identifies particular areas of tobacco genes related to acetylated histones.

The acetylation states of histones current on the upstream, promoter, coding or intronic areas of 88 tobacco genes have been examined with chromatin immunoprecipitation (ChIP) experiments utilizing antibodies that recognised acetylated histone H4.
The DNA sequences enriched within the immunoprecipitates have been amplified by ligation-mediated PCR, labelled with Cy-dUTP and hybridised to DNA microarrays. In inexperienced tobacco shoots, histone H4 acetylation was localised to 300-600-bp sequences within the promoters or coding areas of 31 genes, or occurred extensively over a number of kilobase-pair areas containing the upstream, promoter and/or coding areas of 25 genes.
Genes related to excessive histone H4 acetylation ranges at promoters have been actively expressed, whereas genes depleted in acetylated histone H4 have been non-transcribed or expressed at very low ranges, suggesting a correlation between histone H4 acetylation and gene exercise.
Trichostatin A (TA), an inhibitor of histone deacetylases (HDAs), didn’t alter histone H4 acetylation states globally however elevated acetylation ranges at particular tobacco sequences, suggesting that HDAs are focused to specific nucleosomes. Genes that have been upregulated by TA have been related to elevated histone H4 acetylation at promoter or coding areas, indicating that acetylation of histones on coding areas could activate transcription.
Elevated histone H4 acetylation resulting in elevated expression was noticed on genes with various features, suggesting that histone H4 acetylation is concerned in regulation of many plant processes.

Chromatin profiling by immediately sequencing small portions of immunoprecipitated DNA.

Chromatin construction and transcription issue localization will be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or different properties, however present applied sciences contain a number of steps that introduce bias and inefficiency. Right here we apply a single-molecule strategy to immediately sequence chromatin immunoprecipitated DNA with minimal pattern manipulation. This methodology is suitable with simply 50 pg of DNA and will thus facilitate charting chromatin maps from restricted cell populations.
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