Chromatin – DNA fragments by sonication and immunoprecipitated

Identifying chromatin features that regulate gene expression distribution

Zoe
Zoe
December 11, 2020

Gene expression variability, variations within the variety of mRNA per cell throughout a inhabitants of cells, is ubiquitous throughout various organisms with broad impacts on mobile phenotypes. The position of chromatin in regulating common gene expression has been extensively studied. Nevertheless, what points of the chromatin contribute to gene expression variability remains to be underexplored. Right here we addressed this drawback by leveraging chromatin variety and utilizing a scientific investigation of randomly built-in expression reporters to determine what points of chromatin microenvironment contribute to gene expression variability. Utilizing DNA barcoding and split-pool decoding, we created a big library of isogenic reporter clones and recognized reporter integration websites in a large and parallel method.

By mapping our measurements of reporter expression at completely different genomic loci with a number of epigenetic profiles together with the enrichment of transcription elements and the space to completely different chromatin states, we recognized new elements that influence the regulation of gene expression distributions. Greater-order chromatin construction is tightly linked to gene expression and subsequently cell identification. In recent times, the chromatin panorama of pluripotent stem cells has turn out to be higher characterised, and distinctive options at varied architectural ranges have been revealed.

Nevertheless, the mechanisms that govern institution and upkeep of those topological traits and the temporal and useful relationships with transcriptional or epigenetic options are nonetheless areas of intense research. Right here, we’ll focus on progress and limitations of our present understanding concerning how the 3D chromatin topology of pluripotent stem cells is established throughout somatic cell reprogramming and maintained throughout cell division.

 

Quantification of the effect of site-specific histone acetylation on chromatin transcription rate

Eukaryotic transcription is epigenetically regulated by chromatin construction and post-translational modifications (PTMs). For instance, lysine acetylation in histone H4 is correlated with activation of RNA polymerase I-, II- and III-driven transcription from chromatin templates, which requires prior chromatin reworking. Nevertheless, quantitative understanding of the contribution of specific PTM states to the sequential steps of eukaryotic transcription has been hampered partially as a result of reconstitution of a chromatin template with designed PTMs is troublesome. On this research, we reconstituted a di-nucleosome with site-specifically acetylated or unmodified histone H4, which contained two copies of the Xenopus somatic 5S rRNA gene with addition of a novel sequence detectable by hybridization-assisted fluorescence correlation spectroscopy. Utilizing a Xenopus oocyte nuclear extract, we analyzed the time course of accumulation of nascent 5S rRNA-derived transcripts generated on chromatin templates in vitro.
Our mathematically described kinetic mannequin and becoming evaluation revealed that tetra-acetylation of histone H4 at K5/K8/Ok12/Ok16 will increase the speed of transcriptionally competent chromatin formation ∼3-fold as compared with the absence of acetylation. We offer a kinetic mannequin for quantitative analysis of the contribution of epigenetic modifications to chromatin transcription. The HoxD gene cluster is important for correct limb formation in tetrapods. Within the rising limb buds, completely different subgroups of Hoxd genes reply first to a proximal regulatory sign, then to a distal sign that organizes digits. These two laws are unique from each other and emanate from two distinct topologically associating domains (TADs) flanking HoxD, each containing a spread of applicable enhancer sequences.
The telomeric TAD (T-DOM) comprises a number of enhancers lively in presumptive forearm cells and is split into two sub-TADs separated by a CTCF-rich boundary, which defines two regulatory submodules. To know the significance of this specific regulatory topology to regulate Hoxd gene transcription in time and house, we both deleted or inverted this sub-TAD boundary, eradicated the CTCF binding websites, or inverted all the T-DOM to trade the respective positions of the 2 sub-TADs

An optimised chromatin immunoprecipitation (ChIP) method for starchy leaves of Nicotiana benthamiana to study histone modifications of an allotetraploid plant

All flowering crops have developed by means of a number of rounds of polyploidy all through the evolutionary course of. Intergenomic interactions between subgenomes in polyploid crops are predicted to induce chromatin modifications comparable to histone modifications to control expression of gene homoeologs. Nicotiana benthamiana is an historical allotetraploid plant with ecotypes collected from climatically various areas of Australia. Learning the chromatin panorama of this distinctive assortment will possible make clear the significance of chromatin modifications in gene regulation in polyploids as properly its implications in adaptation of crops in environmentally various situations.
Usually, chromatin immunoprecipitation and excessive throughput DNA sequencing (ChIP-seq) is used to review chromatin modifications. Nevertheless, as a result of starchy nature of mature N. benthamiana leaves, beforehand printed protocols have been unsuitable. The upper quantities of starch in leaves that co-precipitated with nuclei hindered downstream processing of DNA. Right here we current an optimised ChIP protocol for N. benthamiana leaves to facilitate comparability of chromatin modifications in two intently associated ecotypes. A number of steps of ChIP have been optimised together with tissue harvesting, nuclei isolation, nuclei storage, DNA shearing and DNA restoration. Generally obtainable antibodies concentrating on histone Three lysine four trimethylation (H3K4me3) and histone Three lysine 9 dimethylation (H3K9me2) histone modifications have been used and success of ChIP was confirmed by PCR and subsequent era sequencing. Collectively, our optimised methodology is the primary complete ChIP methodology for mature starchy leaves of N. benthamiana to allow research of chromatin panorama on the genome-wide scale.

Chromatin Assembly Factor 1 Subunit A (CHAF1A) Antibody
abx331631-100ul 100 ul
EUR 510

Chromatin Assembly Factor 1 Subunit A (CHAF1A) Antibody
20-abx325413
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Recombinant human Chromatin assembly factor 1 subunit A
P2784 100ug Ask for price
Description: Recombinant protein for human Chromatin assembly factor 1 subunit A

Human Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E01C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E01C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E01C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E07C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E07C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E07C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E06C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E06C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E06C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E08C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E08C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E08C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E09C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E09C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E09C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E04C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E04C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E04C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E02C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E02C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E02C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E03C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E03C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E03C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Chromatin Assembly Factor 1 Subunit A (CHAF1A) ELISA Kit
abx386486-96tests 96 tests
EUR 1093.2

Chicken Chromatin assembly factor 1 subunit A, CHAF1A ELISA KIT
ELI-34635c 96 Tests
EUR 1113.6

Human Chromatin assembly factor 1 subunit A, CHAF1A ELISA KIT
ELI-49276h 96 Tests
EUR 988.8

Mouse Chromatin assembly factor 1 subunit A, Chaf1a ELISA KIT
ELI-10881m 96 Tests
EUR 1038

Guinea pig Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E05C1654-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E05C1654-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Chromatin assembly factor 1 subunit A(CHAF1A) ELISA kit
E05C1654-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Chromatin assembly factor 1 subunit A(CHAF1A) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
DLR-CHAF1B-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
DLR-CHAF1B-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) in samples from tissue homogenates, cell lysates or other biological fluids.

Human Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E01C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E01C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E01C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E07C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E07C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E07C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E06C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E06C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E06C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E08C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E08C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E08C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E09C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E09C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E09C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E04C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E04C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E04C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E02C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E02C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E02C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E03C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E03C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E03C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
20-abx153813
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Chromatin assembly factor 1 subunit B, CHAF1B ELISA KIT
ELI-33019h 96 Tests
EUR 988.8

Mouse Chromatin assembly factor 1 subunit B, Chaf1b ELISA KIT
ELI-50128m 96 Tests
EUR 1038

Chicken Chromatin assembly factor 1 subunit B, CHAF1B ELISA KIT
ELI-25250c 96 Tests
EUR 1113.6

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
RDR-CHAF1B-Mu-48Tests 48 Tests
EUR 668.4

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
RDR-CHAF1B-Mu-96Tests 96 Tests
EUR 928.8

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
RD-CHAF1B-Mu-48Tests 48 Tests
EUR 639.6

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
RD-CHAF1B-Mu-96Tests 96 Tests
EUR 888

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
SEJ447Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) in Tissue homogenates, cell Lysates and other biological fluids.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
SEJ447Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) in Tissue homogenates, cell Lysates and other biological fluids.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
SEJ447Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) in Tissue homogenates, cell Lysates and other biological fluids.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
SEJ447Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) in Tissue homogenates, cell Lysates and other biological fluids.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) ELISA Kit
4-SEJ447Mu
  • EUR 5895.60
  • EUR 3114.00
  • EUR 786.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) in samples from Tissue homogenates, cell Lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Antibody
20-abx175859
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Chromatin Assembly Factor 1 Subunit B (CAF1B) Antibody
20-abx119065
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Antibody
20-abx129436
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Antibody
20-abx004781
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Antibody
abx340034-100ug 100 ug
EUR 469.2

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Antibody
20-abx329402
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Recombinant Chromatin Assembly Factor 1, Subunit B (CHAF1B)
4-RPJ447Mu01
  • EUR 593.09
  • EUR 282.00
  • EUR 1894.08
  • EUR 711.36
  • EUR 1302.72
  • EUR 472.80
  • EUR 4555.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Chromatin Assembly Factor 1, Subunit B expressed in: E.coli

Guinea pig Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E05C1655-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E05C1655-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Chromatin assembly factor 1 subunit B(CHAF1B) ELISA kit
E05C1655-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Chromatin assembly factor 1 subunit B(CHAF1B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Mouse CHAF1B (Chromatin Assembly Factor 1, Subunit B)
ELK6320 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Chromatin Assembly Factor 1, Subunit B from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) CLIA Kit
20-abx495711
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B) Protein
20-abx167366
  • EUR 828.00
  • EUR 343.20
  • EUR 2548.80
  • EUR 978.00
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse)
4-PAJ447Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B)

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse), APC
4-PAJ447Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B). This antibody is labeled with APC.

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse), Biotinylated
4-PAJ447Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B). This antibody is labeled with Biotin.

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse), Cy3
4-PAJ447Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B). This antibody is labeled with Cy3.

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse), FITC
4-PAJ447Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B). This antibody is labeled with FITC.

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse), HRP
4-PAJ447Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B). This antibody is labeled with HRP.

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse), PE
4-PAJ447Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B). This antibody is labeled with PE.

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
ELISA-1 1
EUR 242.4

Bovine Chromatin complexes subunit BAP18, BAP18 ELISA KIT
ELI-11441b 96 Tests
EUR 1113.6

Chromatin Assembly Factor 1, Subunit B (CHAF1B) Polyclonal Antibody (Mouse), APC-Cy7
4-PAJ447Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Chromatin Assembly Factor 1, Subunit B (CHAF1B). This antibody is labeled with APC-Cy7.

Bovine Dynein assembly factor 1, axonemal, DNAAF1 ELISA KIT
ELI-26476b 96 Tests
EUR 1113.6

DNAAF1 ELISA Kit| Bovine Dynein assembly factor 1, axonemal ELI
EF011338 96 Tests
EUR 826.8

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 
P-2002
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

Filtration System 47mm Complete Assembly
5809/KIT EACH
EUR 427.69

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit 
P-2003
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

MBD1 Polyclonal Antibody
A-1006
  • EUR 740.34
  • EUR 117.70
  • EUR 306.90
  • EUR 462.00
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]
A-1014
  • EUR 721.20
  • EUR 82.50
  • EUR 239.80
  • EUR 456.50
  • 100 µg
  • 10 ug
  • 50 ug
  • 100 ug

5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]
A-1018
  • EUR 702.06
  • EUR 82.50
  • EUR 239.80
  • EUR 443.30
  • 100 µg
  • 10 ug
  • 50 ug
  • 100 ug

PRDM4 Polyclonal Antibody
A-2004
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM5 Polyclonal Antibody
A-2005
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM10 Polyclonal Antibody
A-2010
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM11 Polyclonal Antibody
A-2011
  • EUR 754.26
  • Ask for price
  • Ask for price
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM13 Polyclonal Antibody
A-2013
  • EUR 754.26
  • Ask for price
  • Ask for price
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM16 Polyclonal Antibody
A-2016
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM17 Polyclonal Antibody
A-2017
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

EZH1 Polyclonal Antibody
A-2018
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

HSF1 Polyclonal Antibody
A-2401
  • EUR 905.64
  • EUR 235.40
  • EUR 423.50
  • EUR 574.20
  • 200 µl
  • 50 ul
  • 100 ul
  • 200 ul

PRMT3 Polyclonal Antibody
A-3003
  • EUR 740.34
  • Ask for price
  • Ask for price
  • EUR 462.00
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

SET1 Polyclonal Antibody
A-3011
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

SET07 Polyclonal Antibody
A-3013
  • EUR 754.26
  • Ask for price
  • Ask for price
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

LSD1 Polyclonal Antibody
A-3018
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

HDAC10 Polyclonal Antibody
A-4010
  • EUR 754.26
  • Ask for price
  • Ask for price
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PCAF Polyclonal Antibody
A-4012
  • EUR 740.34
  • EUR 117.70
  • EUR 306.90
  • EUR 462.00
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

Histone H3K9ac (Acetyl H3K9) Polyclonal Antibody
A-4022
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K14ac (Acetyl H3K14) Polyclonal Antibody
A-4023
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody
A-4024
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K23ac (Acetyl H3K23) Polyclonal Antibody
A-4025
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 383.90
  • 50 µg
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K56ac (Acetyl H3K56) Polyclonal Antibody
A-4026
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody
A-4031
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody
A-4032
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 383.90
  • 50 µg
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody
A-4033
  • EUR 555.90
  • EUR 177.10
  • EUR 342.10
  • EUR 479.60
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K9me1 (H3K9 Monomethyl) Polyclonal Antibody
A-4034
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K9me2 (H3K9 Dimethyl) Polyclonal Antibody
A-4035
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody
A-4036
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K27me1 (H3K27 Monomethyl) Polyclonal Antibody
A-4037
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K27me2 (H3K27 Dimethyl) Polyclonal Antibody
A-4038
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody
A-4039
  • EUR 555.90
  • EUR 177.10
  • EUR 342.10
  • EUR 479.60
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul
The results of such perturbations on the transcriptional regulation of Hoxd genes illustrate the requirement of this regulatory topology for the exact timing of gene activation. Nevertheless, the spatial distribution of transcripts was ultimately resumed, exhibiting that the presence of enhancer sequences, reasonably than both their actual topology or a specific chromatin structure, is the important thing issue. We additionally present that the affinity of enhancers to search out their pure goal genes can overcome the presence of each a robust TAD border and an unfavorable orientation of CTCF websites.
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