Chromatin – DNA fragments by sonication and immunoprecipitated
chipready

Integrated top-down and bottom-up Proteomic Approach to Characterize The Antigen-Binding

Zoe
Zoe

An integrated top-down and bottom-up proteomic approach to characterize the antigen-binding fragment of antibodies.

We have previously shown that different individuals exposed to the same antigen produce antibodies with identical mutations in their complementarity determining regions (CDR), suggesting that CDR tryptic peptides can serve as biomarkers for disease diagnosis and prognosis.

 

Complete Fabs derived from disease-specific antibodies have even higher potential; they could potentially be used for disease treatment and are required to identify the antigens toward which the antibodies are directed. However, complete Fab sequence characterization via LC-MS analysis of tryptic peptides (i.e. bottom-up) has proven to be impractical for mixtures of antibodies.

 

To tackle this challenge, we have developed an integrated bottom-up and top-down MS approach, employing 2D chromatography coupled with Fourier transform mass spectrometry (FTMS), and applied this approach for full characterization of the variable parts of two pharmaceutical monoclonal antibodies with sensitivity comparable to the bottom-up standard.

 

These efforts represent an essential step toward the identification of disease specific antibodies in patient samples with potentially significant clinical impact.

Selective 351 nm photodissociation of cysteine-containing peptides for discrimination of antigen-binding regions of IgG fragments in bottom-up liquid chromatography-tandem mass spectrometry workflows.

Despite tremendous inroads in the development of more sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategies for mass spectrometry-based proteomics, there remains a significant need for enhancing the selectivity of MS/MS-based workflows for streamlined analysis of complex biological mixtures.

 

Here, a novel LC-MS/MS platform based on 351 nm ultraviolet photodissociation (UVPD) is presented for the selective analysis of cysteine-peptide subsets in complex protein digests. Cysteine-selective UVPD is mediated through the site-specific conjugation of reduced cysteine residues with a 351 nm active chromogenic Alexa Fluor 350 (AF350) maleimide tag.

 

Only peptides containing the AF350 chromophore undergo photodissociation into extensive arrays of b- and y-type fragment ions, thus providing a facile means for differentiating cysteine-peptide targets from convoluting peptide backgrounds.

 

With the use of this approach in addition to strategic proteolysis, the selective analysis of diagnostic heavy-chain complementarity determining regions (CDRs) of single-chain antibody (scAb) fragments is demonstrated.

Fragments of the Roelofs effect: a bottom-up effect equal to the sum of its parts.

The Roelofs effect is a distortion of perceived space that occurs when a large frame whose center is offset left or right from the objective midline is presented visually to an observer and causes a bias in the observer’s subjective judgment of midline.

 

Experiments were designed to test whether an isolated fragment (left or right end) of a Roelofs-inducing frame was capable of generating the Roelofs effect and to determine whether prior experience with intact frames would provide a top-down influence that would bias the Roelofs effect resulting from fragment presentation.

 

Although the fragments did induce an effect, top-down information did not play a significant role even after a 5-day training paradigm. Instead, we found that the effect generated by an intact frame was equal in magnitude to the sum of the effects generated by the individual fragments.

 

In addition, perception was found to be differentially affected by the two ends of the frame, with fragments falling in the right visual field causing a larger effect than those falling in the left.

chipready
chipready

Pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E07R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E08R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E08R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E08R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A16275 96T
EUR 700
Description: ELISA

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A51178 96T
EUR 700
Description: ELISA

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A25022 96T
EUR 700
Description: ELISA

Sheep Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A103472 96T
EUR 700
Description: ELISA

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A7530 96T
EUR 700
Description: ELISA

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A33744 96T
EUR 700
Description: ELISA

Canine Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A68606 96T
EUR 700
Description: ELISA

Bovine Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A86045 96T
EUR 700
Description: ELISA

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A77325 96T
EUR 700
Description: ELISA

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Porcine Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A59896 96T
EUR 700
Description: ELISA

Chicken Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A94757 96T
EUR 700
Description: ELISA

Guinea Pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01A42461 96T
EUR 700
Description: ELISA

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rhizopus Arrhizus mouse monoclonal antibody, clone WSSA-RA-1, Purified

AM05895PU-N 250 µg Ask for price

Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

1-CSB-EP2860XBC
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu(tuf) expressed in E.coli

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

CSB-EP2860XBC 2583 mg Ask for price

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

RPC28227-100ug 100ug
EUR 765.5

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

RPC28227-1mg 1mg
EUR 2959.2

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

RPC28227-20ug 20ug
EUR 448.1

α-Amylase from Aspergillus oryzae

abx071102-100mg 100 mg
EUR 237.5

Recombinant Magnaporthe oryzae Cutinase (CUT1)

CSB-YP333519MPM 10592 mg Ask for price

Recombinant Magnaporthe oryzae Cutinase (CUT1)

CSB-EP333519MPM 10748 mg Ask for price

Recombinant Magnaporthe oryzae Cutinase (CUT1)

CSB-EP333519MPMa0 10749 mg Ask for price

Magnaporthe oryzae Scytalone dehydratase (SDH1)

1-CSB-EP348697MPM
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Magnaporthe oryzae Scytalone dehydratase(SDH1) expressed in E.coli

OORA00214-v1 - Nuclease S1 (Aspergillus oryzae sp.)

OORA00214-v1 10ku
EUR 150

OORA00214-10KU - Nuclease S1 (Aspergillus oryzae sp.)

OORA00214-10KU 10ku
EUR 149

Native Aspergillus oryzae exo-Inulinase (Food Grade)

NATE-1250 1kg
EUR 324

Recombinant Magnaporthe oryzae Scytalone dehydratase(SDH1)

AP73887 1mg
EUR 2826

Recombinant Magnaporthe oryzae Scytalone dehydratase (SDH1)

CSB-EP348697MPM 3158 mg Ask for price

Native Aspergillus oryzae endo-Inulinase (food grade)

NATE-1246 1kg
EUR 324

Recombinant Aspergillus Oryzae H2A Protein (aa 2-133) [His]

VAng-Wyb3980-1mg 1 mg
EUR 4488
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Histone H2A, recombinant protein.

Recombinant Aspergillus Oryzae ACT1 Protein (aa 1-375) [His]

VAng-Wyb3947-1mg 1 mg
EUR 5642.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Actin, recombinant protein.

Recombinant Aspergillus Oryzae ADH5 Protein (aa 1-383) [His]

VAng-Wyb3948-1mg 1 mg
EUR 5676
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Alcohol Dehydrogenase 5 (Class III), chi Polypeptide, recombinant protein.

Recombinant Aspergillus Oryzae Amy3 Protein (aa 22-499) [His]

VAng-Wyb3949-1mg 1 mg
EUR 6130.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) alpha-Amylase A Type-3, recombinant protein.

Recombinant Aspergillus Oryzae ATG3 Protein (aa 1-356) [His]

VAng-Wyb3952-1mg 1 mg
EUR 5550
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Autophagy Related 3 Homolog, recombinant protein.

Recombinant Aspergillus Oryzae ATG5 Protein (aa 1-322) [His]

VAng-Wyb3953-1mg 1 mg
EUR 5392.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Autophagy Related 5 Homolog, recombinant protein.

Recombinant Aspergillus Oryzae BCP1 Protein (aa 1-290) [His]

VAng-Wyb3954-1mg 1 mg
EUR 5236.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Bcp1p, recombinant protein.

Recombinant Aspergillus Oryzae CAP1 Protein (aa 1-273) [His]

VAng-Wyb3956-1mg 1 mg
EUR 5157.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cap1p, recombinant protein.

Recombinant Aspergillus Oryzae CBP4 Protein (aa 1-116) [His]

VAng-Wyb3958-1mg 1 mg
EUR 4412.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cbp4p, recombinant protein.

Recombinant Aspergillus Oryzae CFD1 Protein (aa 1-315) [His]

VAng-Wyb3959-1mg 1 mg
EUR 5359.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cfd1p, recombinant protein.

Recombinant Aspergillus Oryzae CHZ1 Protein (aa 1-110) [His]

VAng-Wyb3960-1mg 1 mg
EUR 4386
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Chz1p, recombinant protein.

Recombinant Aspergillus Oryzae CTU2 Protein (aa 1-361) [His]

VAng-Wyb3962-1mg 1 mg
EUR 5574
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cytosolic Thiouridylase Subunit 2 Homolog, recombinant protein.

Recombinant Aspergillus Oryzae DDI1 Protein (aa 1-402) [His]

VAng-Wyb3963-1mg 1 mg
EUR 5768.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) DNA-Damage Inducible 1 Homolog 1, recombinant protein.

Recombinant Aspergillus Oryzae DML1 Protein (aa 1-493) [His]

VAng-Wyb3964-1mg 1 mg
EUR 6199.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Dml1p, recombinant protein.

Recombinant Aspergillus Oryzae DRE2 Protein (aa 1-313) [His]

VAng-Wyb3966-1mg 1 mg
EUR 5346
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Dre2p, recombinant protein.

Recombinant Aspergillus Oryzae EFG1 Protein (aa 1-320) [His]

VAng-Wyb3967-1mg 1 mg
EUR 5378.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Efg1p, recombinant protein.

Recombinant Aspergillus Oryzae Egd2 Protein (aa 1-202) [His]

VAng-Wyb3968-1mg 1 mg
EUR 4820.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Nascent Polypeptide-Associated Complex Subunit alpha, recombinant protein.

Recombinant Aspergillus Oryzae ESF2 Protein (aa 1-335) [His]

VAng-Wyb3972-1mg 1 mg
EUR 5448
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Esf2p, recombinant protein.

Recombinant Aspergillus Oryzae FIS1 Protein (aa 1-126) [His]

VAng-Wyb3973-1mg 1 mg
EUR 4459.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fis1p, recombinant protein.

Recombinant Aspergillus Oryzae FPR1 Protein (aa 1-116) [His]

VAng-Wyb3974-1mg 1 mg
EUR 4412.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fpr1p, recombinant protein.

Recombinant Aspergillus Oryzae FPR2 Protein (aa 20-134) [His]

VAng-Wyb3975-1mg 1 mg
EUR 4407.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fpr2p, recombinant protein.

Recombinant Aspergillus Oryzae FPR4 Protein (aa 1-470) [His]

VAng-Wyb3976-1mg 1 mg
EUR 6091.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fpr4p, recombinant protein.

Recombinant Aspergillus Oryzae HCR1 Protein (aa 1-265) [His]

VAng-Wyb3981-1mg 1 mg
EUR 5119.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) COenzyme Q (Ubiquinone) Hcr1p, recombinant protein.

Recombinant Aspergillus Oryzae JIP5 Protein (aa 1-417) [His]

VAng-Wyb3982-1mg 1 mg
EUR 5840.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Jip5p, recombinant protein.

Recombinant Aspergillus Oryzae KAE1 Protein (aa 1-358) [His]

VAng-Wyb3983-1mg 1 mg
EUR 5563.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Kae1p, recombinant protein.

Recombinant Aspergillus Oryzae LCL2 Protein (aa 22-1226) [His]

VAng-Wyb3984-1mg 1 mg
EUR 4339.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Lcl2p, recombinant protein.

Recombinant Aspergillus Oryzae LIA1 Protein (aa 1-339) [His]

VAng-Wyb3985-1mg 1 mg
EUR 5470.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Lia1p, recombinant protein.

Recombinant Aspergillus Oryzae LOC1 Protein (aa 1-190) [His]

VAng-Wyb3986-1mg 1 mg
EUR 4765.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) COenzyme Q (Ubiquinone) Loc1p, recombinant protein.

Recombinant Aspergillus Oryzae MDE1 Protein (aa 1-241) [His]

VAng-Wyb3988-1mg 1 mg
EUR 5006.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mde1p, recombinant protein.

Recombinant Aspergillus Oryzae MED6 Protein (aa 1-328) [His]

VAng-Wyb3990-1mg 1 mg
EUR 5421.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mediator Complex Subunit 6, recombinant protein.

Recombinant Aspergillus Oryzae MED7 Protein (aa 1-261) [His]

VAng-Wyb3991-1mg 1 mg
EUR 5101.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mediator Complex Subunit 7, recombinant protein.

Recombinant Aspergillus Oryzae MED8 Protein (aa 1-244) [His]

VAng-Wyb3992-1mg 1 mg
EUR 5022
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mediator Complex Subunit 8, recombinant protein.

Recombinant Aspergillus Oryzae MMM1 Protein (aa 47-494) [His]

VAng-Wyb3994-1mg 1 mg
EUR 5989.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mmm1p, recombinant protein.

Recombinant Aspergillus Oryzae Mri1 Protein (aa 1-381) [His]

VAng-Wyb3996-1mg 1 mg
EUR 5668.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Methylthioribose-1-Phosphate Isomerase Homolog, recombinant protein.

Recombinant Aspergillus Oryzae MZM1 Protein (aa 19-114) [His]

VAng-Wyb3997-1mg 1 mg
EUR 4316.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mzm1p, recombinant protein.

Recombinant Aspergillus Oryzae NUCS Protein (aa 21-287) [His]

VAng-Wyb4000-1mg 1 mg
EUR 5131.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) NucS Gene Product, recombinant protein.

Recombinant Aspergillus Oryzae NUF2 Protein (aa 1-463) [His]

VAng-Wyb4001-1mg 1 mg
EUR 6057.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Spindle Pole Body Protein, recombinant protein.

Induction of cell cycle arrest and apoptosis by tomentosin in hepatocellular carcinoma HepG2 and Huh7 cells.

Tomentosin, a sesquiterpene lactone, is known to possess various biological activities. However, its anticarcinogenic activity against human hepatocellular carcinoma (HCC) cells has not been investigated in detail. Thus, this study aimed to elucidate the cytotoxic mechanism of tomentosin in human HCC cell lines HepG2 and Huh7.

 

WST-1, cell counting, and colony formation assay results showed that treatment with tomentosin decreased the viability and suppressed the proliferation rate of HepG2 and Huh7 cells in a dose- and time-dependent manner. Cell cycle analysis revealed increased population of cells at the SubG1 and G2/M stage, and decreased population of cells at the G0/1 stage in HepG2 and Huh7 cells treated with tomentosin.

 

Annexin V/propidium iodide double staining and TUNEL assay results showed increased apoptotic cell population and DNA fragmentation in HepG2 and Huh7 cells treated with tomentosin.

 

Western blotting analysis results showed that tomentosin treatment significantly increased the expression level of Bax, Bim (short form), cleaved PARP1, FOXO3, p53, pSer15p53, pSer20p53, pSer46p53, p21, and p27, but decreased the expression of Bcl2, caspase3, caspase7, caspase9, cyclin-dependent kinase 2 (CDK2), CDK4, CDK6, cyclinB1, cyclinD1, cyclinD2, cyclinD3, and cyclinE in a dose-dependent manner.

 

Taken together, this study revealed that tomentosin, which acted through cell cycle arrest and apoptosis, may be a useful therapeutic option against HCC.

Keywords: FOXO3; Tomentosin; apoptosis; cell cycle arrest; hepatocellular carcinoma; p53.

 

Fusarium sp.

PCR-VH007-PCRVH00748D PCR-VH007-48D
EUR 230

Fusarium sp.

PCR-VH007-PCRVH00796D PCR-VH007-96D
EUR 312

Fusarium sp. PCR kit

PCR-VH007-48D 50T
EUR 543.6

Fusarium sp. PCR kit

PCR-VH007-96D 100T
EUR 686.4

Fusarium sp. RT PCR kit

RTq-VH007-100D 100T
EUR 860.4

Fusarium sp. RT PCR kit

RTq-VH007-150D 150T
EUR 969.6

Fusarium sp. RT PCR kit

RTq-VH007-50D 50T
EUR 717.6

Fusarium antigen ELISA Kit

DEIABL66 96T
EUR 704
Description: Soil, growing or harvested plants, grain, food, washes from industrial equipment and other types of material

Fusarium sp. One-Step PCR kit

Oneq-VH007-100D 100T
EUR 1039.2

Fusarium sp. One-Step PCR kit

Oneq-VH007-150D 150T
EUR 1177.2

Fusarium sp. One-Step PCR kit

Oneq-VH007-50D 50T
EUR 861.6

Fusarium Species TaqProbe qPCR Detection; 100 RXN

TBS42019-100 100 tests
EUR 398

Fusarium Oxysporum f.sp. Vasinfectum (FOV) TaqProbe qPCR. 100 RXN

TBS42017-100 100 RXN
EUR 1100

Fusarium Oxysporum f.sp. Vasinfectum (FOV) TaqProbe qPCR. 50 RXN

TBS42017-50 50 RXN
EUR 600

genesig Real-time PCR detection kit for Fusarium species

Z-Path-Fusarium-spp 150 tests
EUR 808
Description: Fusarium_spp

genesig Std Real-time PCR detection kit for Fusarium specieS

Z-Path-Fusarium_spp-std 150 tests
EUR 602
Description: Fusarium_spp

genesig Std Real-time PCR detection kit for Fusarium specieS

Z-Path-Fusarium-spp-std 150 tests
EUR 602
Description: Fusarium_spp

Trichothecene-producing Fusarium TaqProbe qPCR Detection; 100 RXN

TBS42018-100 100 tests
EUR 398

Trichothecene-producing Fusarium TaqProbe qPCR Detection; 200 RXN

TBS42018-200 200 tests
EUR 760

Potato Alternaria solani (A. solani) ELISA Kit

abx055826-100g 100 µg Ask for price

Potato Alternaria solani (A. solani) ELISA Kit

abx055826-10g 10 µg
EUR 493.75

Potato Alternaria solani (A. solani) ELISA Kit

abx055826-50g 50 µg Ask for price

genesig Easy kit for Fusarium_spp

Z-Path-Fusarium_spp-EASY 50 tests
EUR 410
Description: Fusarium_spp

Potato Phytophthora infestans (A. solani) ELISA Kit

abx055825-1mg 1 mg
EUR 493.75

Potato Phytophthora infestans (A. solani) ELISA Kit

abx055825-5mg 5 mg Ask for price

QPCR Kit DNA All Fusariu

MOL8928 EACH
EUR 805.98

QPCR Kit DNA All Fusariu Mmix

MOL8930 EACH
EUR 1027.14

Fusaric Acid

C5062-250 250 mg
EUR 72
Description: inhibitor of dopamine β-hydroxylase

Fusaric Acid

C5062-500 500 mg
EUR 130
Description: inhibitor of dopamine β-hydroxylase

Fusaric Acid

F865400 100mg
EUR 431
Description: 536-69-6

Fusarisetin A

F033-0.5MG 0.5 mg
EUR 207.65
Description: C22H31NO5

Fusarisetin A

F033-2.5MG 2.5 mg
EUR 726.13
Description: C22H31NO5

Solanidin

TBW01598 unit Ask for price

α-Solanine

TN1376-10mg 10mg Ask for price
Description: α-Solanine

α-Solanine

TN1376-1g 1g Ask for price
Description: α-Solanine

α-Solanine

TN1376-1mg 1mg Ask for price
Description: α-Solanine

α-Solanine

TN1376-50mg 50mg Ask for price
Description: α-Solanine

α-Solanine

TN1376-5mg 5mg Ask for price
Description: α-Solanine

Solanidine

S682400 10mg
EUR 431
Description: 80-78-4

Solanidine

T12971-10mg 10mg Ask for price
Description: Solanidine

Solanidine

T12971-1g 1g Ask for price
Description: Solanidine

Solanidine

T12971-1mg 1mg Ask for price
Description: Solanidine

Solanidine

T12971-50mg 50mg Ask for price
Description: Solanidine

Solanidine

T12971-5mg 5mg Ask for price
Description: Solanidine

Solanidiene

T13895-10mg 10mg Ask for price
Description: Solanidiene

Solanidiene

T13895-1g 1g Ask for price
Description: Solanidiene

Solanidiene

T13895-1mg 1mg Ask for price
Description: Solanidiene

Solanidiene

T13895-50mg 50mg Ask for price
Description: Solanidiene

Solanidiene

T13895-5mg 5mg Ask for price
Description: Solanidiene

Solanidine-d5 (Major)

S682401 2.5mg
EUR 11200

7-Hydroxy Solanidine

H398675 10mg
EUR 15000
Description: 911476-37-4

(3b)-3-(3-Azidopropoxy)solanid-5-ene

S064550 0.5mg
EUR 2369
Description: 1644551-84-7

(3b)-3-(11-Azido-3,6,9-trioxaundecoxy)solanid-5-ene

A825025 5mg
EUR 3000

3-(11-Biotinyl-3,6,9-trioxaundecoxy)solanidine

B406850 250mg
EUR 7600

Fusarenon X

F865300 1mg
EUR 890
Description: 23255-69-8

Fusarenon-X

540145 1.0mg
EUR 450

Calcium Fusarate

T23848-10mg 10mg Ask for price
Description: Calcium Fusarate

Calcium Fusarate

T23848-1g 1g Ask for price
Description: Calcium Fusarate

Calcium Fusarate

T23848-1mg 1mg Ask for price
Description: Calcium Fusarate

Calcium Fusarate

T23848-50mg 50mg Ask for price
Description: Calcium Fusarate

Calcium Fusarate

T23848-5mg 5mg Ask for price
Description: Calcium Fusarate

Fusarochromanone

A538960 100mg
EUR 21000
Description: 104653-89-6

(R)-Fusarochromanone

F865595 25mg
EUR 15000
Description: 802915-53-3

Fusarochromanone

T24075-10mg 10mg Ask for price
Description: Fusarochromanone

Fusarochromanone

T24075-1g 1g Ask for price
Description: Fusarochromanone

Fusarochromanone

T24075-1mg 1mg Ask for price
Description: Fusarochromanone

Fusarochromanone

T24075-50mg 50mg Ask for price
Description: Fusarochromanone

Fusarochromanone

T24075-5mg 5mg Ask for price
Description: Fusarochromanone

Solanesol

GP3887-1 1
EUR 139.9

Solanesol

GP3887-1G 1 g
EUR 205.2

Solanesol

GP3887-250 250
EUR 59.3

Solanesol

GP3887-250MG 250 mg
EUR 108

Solanesol (~90%)

S676500 25g
EUR 103
Description: 13190-97-1

Solanesol

T3236-10mg 10mg Ask for price
Description: Solanesol

Solanesol

T3236-1g 1g Ask for price
Description: Solanesol

Solanesol

T3236-1mg 1mg Ask for price
Description: Solanesol

Solanesol

T3236-50mg 50mg Ask for price
Description: Solanesol

Solanesol

T3236-5mg 5mg Ask for price
Description: Solanesol

Solanesol

TBW00151 10X20mg
EUR 328.8

Solanezumab

E40YR1197 1 mg
EUR 2960
Description: Available in various conjugation types.

Solanthrene

TBW01545 5mg Ask for price

Solanezumab

abx831170-10nmol 10 nmol
EUR 1187.5

Solanezumab

abx831170-5nmol 5 nmol
EUR 412.5

Solanapyrone B

T123988-10mg 10mg Ask for price
Description: Solanapyrone B

Solanapyrone B

T123988-1g 1g Ask for price
Description: Solanapyrone B

Solanapyrone B

T123988-1mg 1mg Ask for price
Description: Solanapyrone B

Solanapyrone B

T123988-50mg 50mg Ask for price
Description: Solanapyrone B

Solanapyrone B

T123988-5mg 5mg Ask for price
Description: Solanapyrone B

Solanapyrone C

T123989-10mg 10mg Ask for price
Description: Solanapyrone C

Solanapyrone C

T123989-1g 1g Ask for price
Description: Solanapyrone C

Solanapyrone C

T123989-1mg 1mg Ask for price
Description: Solanapyrone C
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