Chromatin – DNA fragments by sonication and immunoprecipitated
chipready

Integrated top-down and bottom-up Proteomic Approach to Characterize The Antigen-Binding

Zoe
Zoe

An integrated top-down and bottom-up proteomic approach to characterize the antigen-binding fragment of antibodies.

We have previously shown that different individuals exposed to the same antigen produce antibodies with identical mutations in their complementarity determining regions (CDR), suggesting that CDR tryptic peptides can serve as biomarkers for disease diagnosis and prognosis.

 

Complete Fabs derived from disease-specific antibodies have even higher potential; they could potentially be used for disease treatment and are required to identify the antigens toward which the antibodies are directed. However, complete Fab sequence characterization via LC-MS analysis of tryptic peptides (i.e. bottom-up) has proven to be impractical for mixtures of antibodies.

 

To tackle this challenge, we have developed an integrated bottom-up and top-down MS approach, employing 2D chromatography coupled with Fourier transform mass spectrometry (FTMS), and applied this approach for full characterization of the variable parts of two pharmaceutical monoclonal antibodies with sensitivity comparable to the bottom-up standard.

 

These efforts represent an essential step toward the identification of disease specific antibodies in patient samples with potentially significant clinical impact.

Selective 351 nm photodissociation of cysteine-containing peptides for discrimination of antigen-binding regions of IgG fragments in bottom-up liquid chromatography-tandem mass spectrometry workflows.

Despite tremendous inroads in the development of more sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategies for mass spectrometry-based proteomics, there remains a significant need for enhancing the selectivity of MS/MS-based workflows for streamlined analysis of complex biological mixtures.

 

Here, a novel LC-MS/MS platform based on 351 nm ultraviolet photodissociation (UVPD) is presented for the selective analysis of cysteine-peptide subsets in complex protein digests. Cysteine-selective UVPD is mediated through the site-specific conjugation of reduced cysteine residues with a 351 nm active chromogenic Alexa Fluor 350 (AF350) maleimide tag.

 

Only peptides containing the AF350 chromophore undergo photodissociation into extensive arrays of b- and y-type fragment ions, thus providing a facile means for differentiating cysteine-peptide targets from convoluting peptide backgrounds.

 

With the use of this approach in addition to strategic proteolysis, the selective analysis of diagnostic heavy-chain complementarity determining regions (CDRs) of single-chain antibody (scAb) fragments is demonstrated.

Fragments of the Roelofs effect: a bottom-up effect equal to the sum of its parts.

The Roelofs effect is a distortion of perceived space that occurs when a large frame whose center is offset left or right from the objective midline is presented visually to an observer and causes a bias in the observer’s subjective judgment of midline.

 

Experiments were designed to test whether an isolated fragment (left or right end) of a Roelofs-inducing frame was capable of generating the Roelofs effect and to determine whether prior experience with intact frames would provide a top-down influence that would bias the Roelofs effect resulting from fragment presentation.

 

Although the fragments did induce an effect, top-down information did not play a significant role even after a 5-day training paradigm. Instead, we found that the effect generated by an intact frame was equal in magnitude to the sum of the effects generated by the individual fragments.

 

In addition, perception was found to be differentially affected by the two ends of the frame, with fragments falling in the right visual field causing a larger effect than those falling in the left.

chipready
chipready

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E06R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E03R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E01R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E04R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E09R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 ELISA kit

E05R0362-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Rhizopus arrhizu Fatty Acid Desaturases 6 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

1-CSB-EP2860XBC
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu(tuf) expressed in E.coli

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

RPC28227-100ug 100ug
EUR 765.5

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

RPC28227-1mg 1mg
EUR 2959.2

Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

RPC28227-20ug 20ug
EUR 448.1

Magnaporthe oryzae Scytalone dehydratase (SDH1)

1-CSB-EP348697MPM
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Magnaporthe oryzae Scytalone dehydratase(SDH1) expressed in E.coli

Native Aspergillus oryzae exo-Inulinase (Food Grade)

NATE-1250 1kg
EUR 324

Native Aspergillus oryzae endo-Inulinase (food grade)

NATE-1246 1kg
EUR 324

Recombinant Aspergillus Oryzae H2A Protein (aa 2-133) [His]

VAng-Wyb3980-1mg 1 mg
EUR 4488
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Histone H2A, recombinant protein.

Recombinant Aspergillus Oryzae ACT1 Protein (aa 1-375) [His]

VAng-Wyb3947-1mg 1 mg
EUR 5642.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Actin, recombinant protein.

Recombinant Aspergillus Oryzae ADH5 Protein (aa 1-383) [His]

VAng-Wyb3948-1mg 1 mg
EUR 5676
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Alcohol Dehydrogenase 5 (Class III), chi Polypeptide, recombinant protein.

Recombinant Aspergillus Oryzae Amy3 Protein (aa 22-499) [His]

VAng-Wyb3949-1mg 1 mg
EUR 6130.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) alpha-Amylase A Type-3, recombinant protein.

Recombinant Aspergillus Oryzae ATG3 Protein (aa 1-356) [His]

VAng-Wyb3952-1mg 1 mg
EUR 5550
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Autophagy Related 3 Homolog, recombinant protein.

Recombinant Aspergillus Oryzae ATG5 Protein (aa 1-322) [His]

VAng-Wyb3953-1mg 1 mg
EUR 5392.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Autophagy Related 5 Homolog, recombinant protein.

Recombinant Aspergillus Oryzae BCP1 Protein (aa 1-290) [His]

VAng-Wyb3954-1mg 1 mg
EUR 5236.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Bcp1p, recombinant protein.

Recombinant Aspergillus Oryzae CAP1 Protein (aa 1-273) [His]

VAng-Wyb3956-1mg 1 mg
EUR 5157.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cap1p, recombinant protein.

Recombinant Aspergillus Oryzae CBP4 Protein (aa 1-116) [His]

VAng-Wyb3958-1mg 1 mg
EUR 4412.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cbp4p, recombinant protein.

Recombinant Aspergillus Oryzae CFD1 Protein (aa 1-315) [His]

VAng-Wyb3959-1mg 1 mg
EUR 5359.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cfd1p, recombinant protein.

Recombinant Aspergillus Oryzae CHZ1 Protein (aa 1-110) [His]

VAng-Wyb3960-1mg 1 mg
EUR 4386
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Chz1p, recombinant protein.

Recombinant Aspergillus Oryzae CTU2 Protein (aa 1-361) [His]

VAng-Wyb3962-1mg 1 mg
EUR 5574
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Cytosolic Thiouridylase Subunit 2 Homolog, recombinant protein.

Recombinant Aspergillus Oryzae DDI1 Protein (aa 1-402) [His]

VAng-Wyb3963-1mg 1 mg
EUR 5768.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) DNA-Damage Inducible 1 Homolog 1, recombinant protein.

Recombinant Aspergillus Oryzae DML1 Protein (aa 1-493) [His]

VAng-Wyb3964-1mg 1 mg
EUR 6199.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Dml1p, recombinant protein.

Recombinant Aspergillus Oryzae DRE2 Protein (aa 1-313) [His]

VAng-Wyb3966-1mg 1 mg
EUR 5346
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Dre2p, recombinant protein.

Recombinant Aspergillus Oryzae EFG1 Protein (aa 1-320) [His]

VAng-Wyb3967-1mg 1 mg
EUR 5378.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Efg1p, recombinant protein.

Recombinant Aspergillus Oryzae Egd2 Protein (aa 1-202) [His]

VAng-Wyb3968-1mg 1 mg
EUR 4820.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Nascent Polypeptide-Associated Complex Subunit alpha, recombinant protein.

Recombinant Aspergillus Oryzae ESF2 Protein (aa 1-335) [His]

VAng-Wyb3972-1mg 1 mg
EUR 5448
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Esf2p, recombinant protein.

Recombinant Aspergillus Oryzae FIS1 Protein (aa 1-126) [His]

VAng-Wyb3973-1mg 1 mg
EUR 4459.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fis1p, recombinant protein.

Recombinant Aspergillus Oryzae FPR1 Protein (aa 1-116) [His]

VAng-Wyb3974-1mg 1 mg
EUR 4412.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fpr1p, recombinant protein.

Recombinant Aspergillus Oryzae FPR2 Protein (aa 20-134) [His]

VAng-Wyb3975-1mg 1 mg
EUR 4407.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fpr2p, recombinant protein.

Recombinant Aspergillus Oryzae FPR4 Protein (aa 1-470) [His]

VAng-Wyb3976-1mg 1 mg
EUR 6091.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Fpr4p, recombinant protein.

Recombinant Aspergillus Oryzae HCR1 Protein (aa 1-265) [His]

VAng-Wyb3981-1mg 1 mg
EUR 5119.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) COenzyme Q (Ubiquinone) Hcr1p, recombinant protein.

Recombinant Aspergillus Oryzae JIP5 Protein (aa 1-417) [His]

VAng-Wyb3982-1mg 1 mg
EUR 5840.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Jip5p, recombinant protein.

Recombinant Aspergillus Oryzae KAE1 Protein (aa 1-358) [His]

VAng-Wyb3983-1mg 1 mg
EUR 5563.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Kae1p, recombinant protein.

Recombinant Aspergillus Oryzae LCL2 Protein (aa 22-1226) [His]

VAng-Wyb3984-1mg 1 mg
EUR 4339.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Lcl2p, recombinant protein.

Recombinant Aspergillus Oryzae LIA1 Protein (aa 1-339) [His]

VAng-Wyb3985-1mg 1 mg
EUR 5470.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Lia1p, recombinant protein.

Recombinant Aspergillus Oryzae LOC1 Protein (aa 1-190) [His]

VAng-Wyb3986-1mg 1 mg
EUR 4765.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) COenzyme Q (Ubiquinone) Loc1p, recombinant protein.

Recombinant Aspergillus Oryzae MDE1 Protein (aa 1-241) [His]

VAng-Wyb3988-1mg 1 mg
EUR 5006.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mde1p, recombinant protein.

Recombinant Aspergillus Oryzae MED6 Protein (aa 1-328) [His]

VAng-Wyb3990-1mg 1 mg
EUR 5421.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mediator Complex Subunit 6, recombinant protein.

Recombinant Aspergillus Oryzae MED7 Protein (aa 1-261) [His]

VAng-Wyb3991-1mg 1 mg
EUR 5101.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mediator Complex Subunit 7, recombinant protein.

Recombinant Aspergillus Oryzae MED8 Protein (aa 1-244) [His]

VAng-Wyb3992-1mg 1 mg
EUR 5022
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mediator Complex Subunit 8, recombinant protein.

Recombinant Aspergillus Oryzae MMM1 Protein (aa 47-494) [His]

VAng-Wyb3994-1mg 1 mg
EUR 5989.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mmm1p, recombinant protein.

Recombinant Aspergillus Oryzae Mri1 Protein (aa 1-381) [His]

VAng-Wyb3996-1mg 1 mg
EUR 5668.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Methylthioribose-1-Phosphate Isomerase Homolog, recombinant protein.

Recombinant Aspergillus Oryzae MZM1 Protein (aa 19-114) [His]

VAng-Wyb3997-1mg 1 mg
EUR 4316.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mzm1p, recombinant protein.

Recombinant Aspergillus Oryzae NUCS Protein (aa 21-287) [His]

VAng-Wyb4000-1mg 1 mg
EUR 5131.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) NucS Gene Product, recombinant protein.

Recombinant Aspergillus Oryzae NUF2 Protein (aa 1-463) [His]

VAng-Wyb4001-1mg 1 mg
EUR 6057.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Spindle Pole Body Protein, recombinant protein.

Recombinant Aspergillus Oryzae P4HB Protein (aa 21-515) [His]

VAng-Wyb4002-1mg 1 mg
EUR 6210
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Prolyl 4-Hydroxylase, beta Polypeptide, recombinant protein.

Recombinant Aspergillus Oryzae PNO1 Protein (aa 1-260) [His]

VAng-Wyb4003-1mg 1 mg
EUR 5095.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Pno1p, recombinant protein.

Recombinant Aspergillus Oryzae POA1 Protein (aa 1-201) [His]

VAng-Wyb4004-1mg 1 mg
EUR 4818
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Poa1p, recombinant protein.

Recombinant Aspergillus Oryzae PXR1 Protein (aa 1-306) [His]

VAng-Wyb4006-1mg 1 mg
EUR 5312.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Pxr1p, recombinant protein.

Recombinant Aspergillus Oryzae RMT2 Protein (aa 1-413) [His]

VAng-Wyb4008-1mg 1 mg
EUR 5817.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Rmt2p, recombinant protein.

Recombinant Aspergillus Oryzae RPS1 Protein (aa 2-256) [His]

VAng-Wyb4009-1mg 1 mg
EUR 5072.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Rps1 Gene Product, recombinant protein.

Recombinant Aspergillus Oryzae RRD1 Protein (aa 1-478) [His]

VAng-Wyb4010-1mg 1 mg
EUR 6130.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Rrd1p, recombinant protein.

Recombinant Aspergillus Oryzae RRD2 Protein (aa 1-422) [His]

VAng-Wyb4011-1mg 1 mg
EUR 5864.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Rrd2p, recombinant protein.

Recombinant Aspergillus Oryzae RRG9 Protein (aa 54-284) [His]

VAng-Wyb4012-1mg 1 mg
EUR 4959.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Rrg9p, recombinant protein.

Recombinant Aspergillus Oryzae SFH5 Protein (aa 1-455) [His]

VAng-Wyb4014-1mg 1 mg
EUR 6018
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Sfh5p, recombinant protein.

Recombinant Aspergillus Oryzae Slx1 Protein (aa 1-406) [His]

VAng-Wyb4015-1mg 1 mg
EUR 5791.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Structure-Specific Endonuclease Subunit Slx1, recombinant protein.

Recombinant Aspergillus Oryzae SNX3 Protein (aa 1-142) [His]

VAng-Wyb4016-1mg 1 mg
EUR 4537.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Sorting Nexin 3, recombinant protein.

Recombinant Aspergillus Oryzae SOD1 Protein (aa 2-154) [His]

VAng-Wyb4017-1mg 1 mg
EUR 4591.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Triosephosphate Isomerase 1, recombinant protein.

Recombinant Aspergillus Oryzae srb5 Protein (aa 1-273) [His]

VAng-Wyb4018-1mg 1 mg
EUR 5157.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Mediator of RNA polymerase II transcription subunit 18, recombinant protein.

Recombinant Aspergillus Oryzae SSN8 Protein (aa 1-301) [His]

VAng-Wyb4019-1mg 1 mg
EUR 5289.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) COenzyme Q (Ubiquinone) Ssn8p, recombinant protein.

Recombinant Aspergillus Oryzae STS1 Protein (aa 1-312) [His]

VAng-Wyb4021-1mg 1 mg
EUR 5338.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Sts1p, recombinant protein.

Recombinant Aspergillus Oryzae TEF1 Protein (aa 1-460) [His]

VAng-Wyb4022-1mg 1 mg
EUR 6045.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Tef1p, recombinant protein.

Recombinant Aspergillus Oryzae TPI1 Protein (aa 1-251) [His]

VAng-Wyb4024-1mg 1 mg
EUR 5048.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Triosephosphate Isomerase 1, recombinant protein.

Recombinant Aspergillus Oryzae UBA4 Protein (aa 1-463) [His]

VAng-Wyb4026-1mg 1 mg
EUR 6057.6
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) COenzyme Q (Ubiquinone) Uba4p, recombinant protein.

Recombinant Aspergillus Oryzae Urm1 Protein (aa 1-120) [His]

VAng-Wyb4027-1mg 1 mg
EUR 4428
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Urm1, recombinant protein.

Recombinant Aspergillus Oryzae XLNA Protein (aa 20-232) [His]

VAng-Wyb4028-1mg 1 mg
EUR 4874.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) XlnA Gene Product, recombinant protein.

Recombinant Aspergillus Oryzae XlnB Protein (aa 20-221) [His]

VAng-Wyb4029-1mg 1 mg
EUR 4820.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Probable Endo-1,4-beta-Xylanase B, recombinant protein.

Recombinant Aspergillus Oryzae ATG12 Protein (aa 1-176) [His]

VAng-Wyb3951-1mg 1 mg
EUR 4699.2
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Autophagy Related 12, recombinant protein.

Recombinant Aspergillus Oryzae BUD32 Protein (aa 1-272) [His]

VAng-Wyb3955-1mg 1 mg
EUR 5150.4
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Bud32p, recombinant protein.

Recombinant Aspergillus Oryzae DOT1L Protein (aa 1-335) [His]

VAng-Wyb3965-1mg 1 mg
EUR 5448
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) DOT1-Like, Histone H3 Methyltransferase, recombinant protein.

Recombinant Aspergillus Oryzae EIF3E Protein (aa 1-451) [His]

VAng-Wyb3969-1mg 1 mg
EUR 5998.8
Description: Aspergillus Oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) Eukaryotic Translation Initiation Factor 3 Subunit E, recombinant protein.

Induction of cell cycle arrest and apoptosis by tomentosin in hepatocellular carcinoma HepG2 and Huh7 cells.

Tomentosin, a sesquiterpene lactone, is known to possess various biological activities. However, its anticarcinogenic activity against human hepatocellular carcinoma (HCC) cells has not been investigated in detail. Thus, this study aimed to elucidate the cytotoxic mechanism of tomentosin in human HCC cell lines HepG2 and Huh7.

 

WST-1, cell counting, and colony formation assay results showed that treatment with tomentosin decreased the viability and suppressed the proliferation rate of HepG2 and Huh7 cells in a dose- and time-dependent manner. Cell cycle analysis revealed increased population of cells at the SubG1 and G2/M stage, and decreased population of cells at the G0/1 stage in HepG2 and Huh7 cells treated with tomentosin.

 

Annexin V/propidium iodide double staining and TUNEL assay results showed increased apoptotic cell population and DNA fragmentation in HepG2 and Huh7 cells treated with tomentosin.

 

Western blotting analysis results showed that tomentosin treatment significantly increased the expression level of Bax, Bim (short form), cleaved PARP1, FOXO3, p53, pSer15p53, pSer20p53, pSer46p53, p21, and p27, but decreased the expression of Bcl2, caspase3, caspase7, caspase9, cyclin-dependent kinase 2 (CDK2), CDK4, CDK6, cyclinB1, cyclinD1, cyclinD2, cyclinD3, and cyclinE in a dose-dependent manner.

 

Taken together, this study revealed that tomentosin, which acted through cell cycle arrest and apoptosis, may be a useful therapeutic option against HCC.

Keywords: FOXO3; Tomentosin; apoptosis; cell cycle arrest; hepatocellular carcinoma; p53.

 

Fusarium sp. PCR kit

PCR-VH007-48D 50T
EUR 543.6

Fusarium sp. PCR kit

PCR-VH007-96D 100T
EUR 686.4

Fusarium sp. RT PCR kit

RTq-VH007-100D 100T
EUR 860.4

Fusarium sp. RT PCR kit

RTq-VH007-150D 150T
EUR 969.6

Fusarium sp. RT PCR kit

RTq-VH007-50D 50T
EUR 717.6

Fusarium sp. One-Step PCR kit

Oneq-VH007-100D 100T
EUR 1039.2

Fusarium sp. One-Step PCR kit

Oneq-VH007-150D 150T
EUR 1177.2

Fusarium sp. One-Step PCR kit

Oneq-VH007-50D 50T
EUR 861.6

QPCR Kit DNA All Fusariu

MOL8928 EACH
EUR 805.98

QPCR Kit DNA All Fusariu Mmix

MOL8930 EACH
EUR 1027.14

Fusaric Acid

C5062-250 250 mg
EUR 174
Description: Fusaric acid is a mycotoxin produced by several species of Fusarium [1]. Mycotoxins are biologically active secondary fungal metabolites found as contaminants of food- and feedstuff. Mycotoxin is capable of causing disease and death in both humans and animals.

Fusaric Acid

C5062-500 500 mg
EUR 268.8
Description: Fusaric acid is a mycotoxin produced by several species of Fusarium [1]. Mycotoxins are biologically active secondary fungal metabolites found as contaminants of food- and feedstuff. Mycotoxin is capable of causing disease and death in both humans and animals.

Fusarisetin A

F033-0.5MG 0.5 mg
EUR 381.6

Fusarisetin A

F033-2.5MG 2.5 mg
EUR 1248

Solanidin

TBW01598 unit Ask for price

Solanesol

GP3887-1G 1 g
EUR 205.2

Solanesol

GP3887-250MG 250 mg
EUR 108

Solanesol

GP3887-1 1
EUR 139.9

Solanesol

GP3887-250 250
EUR 59.3

Solanesol

TBW00151 10X20mg
EUR 328.8

Solanthrene

TBW01545 5mg Ask for price

Solanezumab

E40YR1197 1 mg
EUR 2960
Description: Available in various conjugation types.

Solanogantine

TBZ1911 unit Ask for price

Anti-APP (Solanezumab)-MC-MMAF ADC

ADC-W-700 1mg Ask for price
Description: This ADC product is comprised of an anti-APP monoclonal antibody conjugated via a MC linker to MMAF

Solanum tuberosum Lectin (STA/STL) - Cy3

21761142-1 -
EUR 134.51

Solanum tuberosum Lectin (STA/STL) - Cy3

21761142-2 -
EUR 213.58

Solanum tuberosum Lectin (STA/STL) - Cy5

21761143-1 -
EUR 134.51

Anti-APP (Solanezumab)-SMCC-DM1 ADC

ADC-W-698 1mg Ask for price
Description: This ADC product is comprised of an anti-APP monoclonal antibody conjugated via a SMCC linker to DM1

Anti-APP (Solanezumab)-SPDB-DM4 ADC

ADC-W-699 1mg Ask for price
Description: This ADC product is comprised of an anti-APP monoclonal antibody conjugated via a SPDB linker to DM4

Solanum tuberosum Lectin (STA/STL) - Pure

21510110-1 -
EUR 166.14

Solanum tuberosum Lectin (STA/STL) - Pure

21510110-2 -
EUR 249.22

Solanum tuberosum Lectin (STA/STL) - Texas Red

21761056-1 -
EUR 207.54

Solanum tuberosum Lectin (STA/STL) - DyLight 488

21500048-1 -
EUR 211.07

Anti-APP (Solanezumab)-MC-Vc-PAB-MMAE ADC

ADC-W-701 1mg Ask for price
Description: This ADC product is comprised of an anti-APP monoclonal antibody conjugated via a MC-Vc linker to MMAE

Anti-APP (Solanezumab)-MC-Vc-PAB-SN38 ADC

ADC-W-702 1mg Ask for price
Description: This ADC product is comprised of an anti-APP monoclonal antibody conjugated via a MC-Vc-PAB linker to SN38

Solanum tuberosum Lectin (STA/STL) - Ferritin

21510548-1 -
EUR 154.66

Solanum tuberosum Lectin (STA/STL) - Semi-Pure

21511401-1 -
EUR 107.66

Solanum tuberosum Lectin (STA/STL) - Separopore® 4B

20120067-1 -
EUR 72.29

Solanum tuberosum Lectin (STA/STL) - Separopore® 4B

20120067-2 -
EUR 134.51

Solanum tuberosum Lectin (STA/STL) - Macrobeads

21511188-1 -
EUR 129.18

Solanum tuberosum Lectin (STA/STL) - Macrobeads

21511188-2 -
EUR 183.11

Solanum tuberosum Lectin (STA/STL) - Colloidal Gold

21510848-1 -
EUR 163.79

Solanum tuberosum Lectin (STA/STL) - Biotinylated

21510112-1 -
EUR 142.41

Solanum tuberosum Lectin (STA/STL) - TRITC (Rhodamine)

21761055-1 -
EUR 127.19

Solanum tuberosum Lectin (STA/STL) - FITC (Fluorescein)

21761054-1 -
EUR 116.13

Z433927330

HY-126074 10mg
EUR 817.2

Solanum tuberosum Lectin (STA/STL) - AP (Alkaline Phosphatase)

21510750-1 -
EUR 145.5

Solanum tuberosum Lectin (STA/STL) - Separopore® 4B MicroPass™

21511189-1 -
EUR 136.34

Anti-Amyloid Beta Reference Antibody (solanezumab)

E24CHB107 100 μg
EUR 225
Description: Available in various conjugation types.

LL Z1640-2

L038-1MG 1 mg
EUR 181.2

LL Z1640-2

L038-5MG 5 mg
EUR 544.8

LL Z1640-4

L039-1MG 1 mg
EUR 268.8

LL Z1640-4

L039-5MG 5 mg
EUR 849.6

Anti-APP (Solanezumab)-MC-Vc-PAB-DMEA-(PEG2)-duocarmycin SA ADC

ADC-W-703 1mg Ask for price
Description: This ADC product is comprised of an anti-APP monoclonal antibody conjugated via a MC-Vc-PAB-DMEA-(PEG2) linker to duocarmycin SA

Solanum tuberosum Lectin (STA/STL) - Separopore® 4B OnePASS™ Column

21511187-1 -
EUR 136.34

Solanum tuberosum Lectin (STA/STL) - HRP (Horseradish Peroxidase)

21510936-1 -
EUR 146.52

CE(20:5(5Z,8Z,11Z,14Z,17Z)

HY-113463 1mg
EUR 349.2

Solanum tuberosum Lectin (STA/STL) - Separopore® 6B SuperTrap™ Cartridge

20120077-1 -
EUR 90.31

Solanum tuberosum Lectin (STA/STL) - Separopore® 6B SuperTrap™ Cartridge

20120077-2 -
EUR 171.57

Solanum tuberosum Lectin (STA/STL) - Separopore® 6B SuperTrap™ Cartridge

20120077-3 -
EUR 168.92

Solanum tuberosum Lectin (STA/STL) - Separopore® 6B SuperTrap™ Cartridge

20120077-4 -
EUR 273.24

Z-321

HY-19123 10mg
EUR 2047.2

Recombinant Solanum melongena Anthocyanidin 3-O-glucosyltransferase (GT)

RPC27237-100ug 100ug
EUR 1544.5

Recombinant Solanum melongena Anthocyanidin 3-O-glucosyltransferase (GT)

RPC27237-20ug 20ug
EUR 580.8

Z876P rabbit pAb

E28PN5071 100μl
EUR 255
Description: Available in various conjugation types.

Z286A rabbit pAb

E28PT6312 100μl
EUR 255
Description: Available in various conjugation types.

Z324B rabbit pAb

E28PT6817 100μl
EUR 255
Description: Available in various conjugation types.

Z658B rabbit pAb

E28PT7458 100μl
EUR 255
Description: Available in various conjugation types.

Z354A rabbit pAb

E28PT7572 100μl
EUR 255
Description: Available in various conjugation types.

Z585A rabbit pAb

E28PN3372 100μl
EUR 255
Description: Available in various conjugation types.

Z585B rabbit pAb

E28PN3373 100μl
EUR 255
Description: Available in various conjugation types.

Z705A rabbit pAb

E28PN3715 100μl
EUR 255
Description: Available in various conjugation types.

Z804A rabbit pAb

E28PN4168 100μl
EUR 255
Description: Available in various conjugation types.

Z385B Antibody

abx032617-400ul 400 ul
EUR 627.6

Z385B Antibody

abx032617-80l 80 µl
EUR 343.2

Z385B (N-term) Rabbit pAb

E2615600 100ul
EUR 225
Description: Available in various conjugation types.

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276)

31037 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli O157:H7 (Z3276) PMAxx

31037-X 1kit
EUR 513.6
Description: Minimum order quantity: 1 unit of 1kit

Pancreas Tumor Tissue Array - 20 cases of pancreatic cancers and 4 cases of normal pancreatic tissues in duplicates, non-overlapping with Z7020090, with grading and TNM staging data.

Z7020109 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

13Z,16Z-Docosadienoic Acid

C3385-10 10 mg
EUR 351.6
Description: 13Z,16Z-Docosadienoic acid is a natural ?-6 polyunsaturated fatty acid (PUFA) formed through a 2-carbon chain elongation of arachi donic acid. 13Z,16Z-Docosadienoic acid acts as an agonist of free fatty acid receptor 4 (FFAR4, also known as GPR120) [1].

13Z,16Z-Docosadienoic Acid

C3385-5 5 mg
EUR 216
Description: 13Z,16Z-Docosadienoic acid is a natural ?-6 polyunsaturated fatty acid (PUFA) formed through a 2-carbon chain elongation of arachi donic acid. 13Z,16Z-Docosadienoic acid acts as an agonist of free fatty acid receptor 4 (FFAR4, also known as GPR120) [1].

Z280B Polyclonal Antibody

ABP60948-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of Z280B from Human . This Z280B antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Z280B protein

Z280B Polyclonal Antibody

ABP60948-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of Z280B from Human . This Z280B antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Z280B protein

Z280B Polyclonal Antibody

ABP60948-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of Z280B from Human . This Z280B antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Z280B protein

Z280B Polyclonal Antibody

ES10322-100ul 100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against Z280B from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Z280B Polyclonal Antibody

ES10322-50ul 50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against Z280B from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Z280B Polyclonal Antibody

E11-201376 100ug/100ul
EUR 225
Description: Available in various conjugation types.

Adapter pack for 5ml tubes in Z207-A rotors, 2/pk.

Z326-15-A5 1 each
EUR 161.8

Adapter pack for 7ml tubes in Z207-A rotors, 2/pk.

Z326-15-A7 1 each
EUR 161.8

Rat Olfactory receptor 10Z1(OR10Z1) ELISA kit

E02O0394-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Olfactory receptor 10Z1(OR10Z1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Olfactory receptor 10Z1(OR10Z1) ELISA kit

E02O0394-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Olfactory receptor 10Z1(OR10Z1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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