Chromatin – DNA fragments by sonication and immunoprecipitated
chipready

Integrated top-down and bottom-up Proteomic Approach to Characterize The Antigen-Binding

Zoe
Zoe
August 22, 2020

An integrated top-down and bottom-up proteomic approach to characterize the antigen-binding fragment of antibodies.

We have previously shown that different individuals exposed to the same antigen produce antibodies with identical mutations in their complementarity determining regions (CDR), suggesting that CDR tryptic peptides can serve as biomarkers for disease diagnosis and prognosis.

 

Complete Fabs derived from disease-specific antibodies have even higher potential; they could potentially be used for disease treatment and are required to identify the antigens toward which the antibodies are directed. However, complete Fab sequence characterization via LC-MS analysis of tryptic peptides (i.e. bottom-up) has proven to be impractical for mixtures of antibodies.

 

To tackle this challenge, we have developed an integrated bottom-up and top-down MS approach, employing 2D chromatography coupled with Fourier transform mass spectrometry (FTMS), and applied this approach for full characterization of the variable parts of two pharmaceutical monoclonal antibodies with sensitivity comparable to the bottom-up standard.

 

These efforts represent an essential step toward the identification of disease specific antibodies in patient samples with potentially significant clinical impact.

Selective 351 nm photodissociation of cysteine-containing peptides for discrimination of antigen-binding regions of IgG fragments in bottom-up liquid chromatography-tandem mass spectrometry workflows.

Despite tremendous inroads in the development of more sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategies for mass spectrometry-based proteomics, there remains a significant need for enhancing the selectivity of MS/MS-based workflows for streamlined analysis of complex biological mixtures.

 

Here, a novel LC-MS/MS platform based on 351 nm ultraviolet photodissociation (UVPD) is presented for the selective analysis of cysteine-peptide subsets in complex protein digests. Cysteine-selective UVPD is mediated through the site-specific conjugation of reduced cysteine residues with a 351 nm active chromogenic Alexa Fluor 350 (AF350) maleimide tag.

 

Only peptides containing the AF350 chromophore undergo photodissociation into extensive arrays of b- and y-type fragment ions, thus providing a facile means for differentiating cysteine-peptide targets from convoluting peptide backgrounds.

 

With the use of this approach in addition to strategic proteolysis, the selective analysis of diagnostic heavy-chain complementarity determining regions (CDRs) of single-chain antibody (scAb) fragments is demonstrated.

Fragments of the Roelofs effect: a bottom-up effect equal to the sum of its parts.

The Roelofs effect is a distortion of perceived space that occurs when a large frame whose center is offset left or right from the objective midline is presented visually to an observer and causes a bias in the observer’s subjective judgment of midline.

 

Experiments were designed to test whether an isolated fragment (left or right end) of a Roelofs-inducing frame was capable of generating the Roelofs effect and to determine whether prior experience with intact frames would provide a top-down influence that would bias the Roelofs effect resulting from fragment presentation.

 

Although the fragments did induce an effect, top-down information did not play a significant role even after a 5-day training paradigm. Instead, we found that the effect generated by an intact frame was equal in magnitude to the sum of the effects generated by the individual fragments.

 

In addition, perception was found to be differentially affected by the two ends of the frame, with fragments falling in the right visual field causing a larger effect than those falling in the left.

chipready
chipready

Recombinant Rhizopus oryzae Cytochrome b (cob), partial
MBS7079456-INQUIRE INQUIRE Ask for price

Recombinant Rhizopus oryzae FK506-binding protein 2B (FKBP3)
MBS1235608-INQUIRE INQUIRE Ask for price

Recombinant Rhizopus oryzae FK506-binding protein 1 (FKBP1)
MBS1238540-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1030

Recombinant Rhizopus oryzae FK506-binding protein 1 (FKBP1)
MBS1238540-002mgEColi 0.02mg(E-Coli)
EUR 605

Recombinant Rhizopus oryzae FK506-binding protein 1 (FKBP1)
MBS1238540-002mgYeast 0.02mg(Yeast)
EUR 785

Recombinant Rhizopus oryzae FK506-binding protein 1 (FKBP1)
MBS1238540-01mgEColi 0.1mg(E-Coli)
EUR 705

Recombinant Rhizopus oryzae FK506-binding protein 1 (FKBP1)
MBS1238540-01mgYeast 0.1mg(Yeast)
EUR 915

Recombinant Rhizopus oryzae FK506-binding protein 4 (FKBP4)
MBS1178969-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1255

Recombinant Rhizopus oryzae FK506-binding protein 4 (FKBP4)
MBS1178969-002mgEColi 0.02mg(E-Coli)
EUR 925

Recombinant Rhizopus oryzae FK506-binding protein 4 (FKBP4)
MBS1178969-002mgYeast 0.02mg(Yeast)
EUR 1045

Recombinant Rhizopus oryzae FK506-binding protein 4 (FKBP4)
MBS1178969-01mgEColi 0.1mg(E-Coli)
EUR 1110

Recombinant Rhizopus oryzae FK506-binding protein 4 (FKBP4)
MBS1178969-01mgYeast 0.1mg(Yeast)
EUR 1190

Recombinant Rhizopus oryzae FK506-binding protein 5 (FKBP5)
MBS1156059-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1260

Recombinant Rhizopus oryzae FK506-binding protein 5 (FKBP5)
MBS1156059-002mgEColi 0.02mg(E-Coli)
EUR 925

Recombinant Rhizopus oryzae FK506-binding protein 5 (FKBP5)
MBS1156059-002mgYeast 0.02mg(Yeast)
EUR 1050

Recombinant Rhizopus oryzae FK506-binding protein 5 (FKBP5)
MBS1156059-01mgEColi 0.1mg(E-Coli)
EUR 1115

Recombinant Rhizopus oryzae FK506-binding protein 5 (FKBP5)
MBS1156059-01mgYeast 0.1mg(Yeast)
EUR 1195

Recombinant Rhizopus oryzae FK506-binding protein 2A (FKBP2)
MBS1077988-INQUIRE INQUIRE Ask for price

Recombinant Rhizopus oryzae FK506-binding protein 2A (FKBP2)
MBS7014832-002mg 0.02mg
EUR 1420

Recombinant Rhizopus oryzae FK506-binding protein 2A (FKBP2)
MBS7014832-01mg 0.1mg
EUR 2385

Recombinant Rhizopus oryzae FK506-binding protein 2A (FKBP2)
MBS7014832-5x01mg 5x0.1mg
EUR 10680

Recombinant Rhizopus oryzae FK506-binding protein 2B (FKBP3)
MBS7014834-002mg 0.02mg
EUR 1475

Recombinant Rhizopus oryzae FK506-binding protein 2B (FKBP3)
MBS7014834-01mg 0.1mg
EUR 2435

Recombinant Rhizopus oryzae FK506-binding protein 2B (FKBP3)
MBS7014834-5x01mg 5x0.1mg
EUR 10915

Recombinant Rhizopus oryzae L-lactate dehydrogenase B (LDHB)
MBS1333447-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1180

Recombinant Rhizopus oryzae L-lactate dehydrogenase B (LDHB)
MBS1333447-002mgEColi 0.02mg(E-Coli)
EUR 825

Recombinant Rhizopus oryzae L-lactate dehydrogenase B (LDHB)
MBS1333447-002mgYeast 0.02mg(Yeast)
EUR 970

Recombinant Rhizopus oryzae L-lactate dehydrogenase B (LDHB)
MBS1333447-01mgEColi 0.1mg(E-Coli)
EUR 990

Recombinant Rhizopus oryzae L-lactate dehydrogenase B (LDHB)
MBS1333447-01mgYeast 0.1mg(Yeast)
EUR 1135

Recombinant Rhizopus oryzae L-lactate dehydrogenase A (LDHA)
MBS1438928-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1195

Recombinant Rhizopus oryzae L-lactate dehydrogenase A (LDHA)
MBS1438928-002mgEColi 0.02mg(E-Coli)
EUR 850

Recombinant Rhizopus oryzae L-lactate dehydrogenase A (LDHA)
MBS1438928-002mgYeast 0.02mg(Yeast)
EUR 990

Recombinant Rhizopus oryzae L-lactate dehydrogenase A (LDHA)
MBS1438928-01mgEColi 0.1mg(E-Coli)
EUR 1020

Recombinant Rhizopus oryzae L-lactate dehydrogenase A (LDHA)
MBS1438928-01mgYeast 0.1mg(Yeast)
EUR 1125

Recombinant Rhizopus oryzae Orotidine 5'-phosphate decarboxylase (pyrG)
MBS1324181-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1145

Recombinant Rhizopus oryzae Orotidine 5'-phosphate decarboxylase (pyrG)
MBS1324181-002mgEColi 0.02mg(E-Coli)
EUR 780

Recombinant Rhizopus oryzae Orotidine 5'-phosphate decarboxylase (pyrG)
MBS1324181-002mgMammalianCell 0.02mg(Mammalian-Cell)
EUR 1410

Recombinant Rhizopus oryzae Orotidine 5'-phosphate decarboxylase (pyrG)
MBS1324181-01mgBaculovirus 0.1mg(Baculovirus)
EUR 1645

Recombinant Rhizopus oryzae Orotidine 5'-phosphate decarboxylase (pyrG)
MBS1324181-01mgEColi 0.1mg(E-Coli)
EUR 935

Recombinant Rhizopus oryzae FK506-binding protein 2A (FKBP2), partial
MBS7059116-INQUIRE INQUIRE Ask for price

Recombinant Rhizopus oryzae FK506-binding protein 2B (FKBP3), partial
MBS7059130-INQUIRE INQUIRE Ask for price

Recombinant Rhizopus oryzae ATP synthase subunit 9, mitochondrial (atp9)
MBS7008546-002mg 0.02mg
EUR 1335

Recombinant Rhizopus oryzae ATP synthase subunit 9, mitochondrial (atp9)
MBS7008546-01mg 0.1mg
EUR 2300

Recombinant Rhizopus oryzae ATP synthase subunit 9, mitochondrial (atp9)
MBS7008546-5x01mg 5x0.1mg
EUR 10300

Recombinant Rhizopus oryzae Peptidyl-prolyl isomerase cwc27 (cwc27), partial
MBS1162708-INQUIRE INQUIRE Ask for price

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B1 (cyp8)
MBS1221211-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1105

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B1 (cyp8)
MBS1221211-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B1 (cyp8)
MBS1221211-002mgYeast 0.02mg(Yeast)
EUR 855

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B1 (cyp8)
MBS1221211-01mgEColi 0.1mg(E-Coli)
EUR 815

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B1 (cyp8)
MBS1221211-01mgYeast 0.1mg(Yeast)
EUR 1005

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase D (cyp12)
MBS1205200-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1240

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase D (cyp12)
MBS1205200-002mgEColi 0.02mg(E-Coli)
EUR 900

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase D (cyp12)
MBS1205200-002mgYeast 0.02mg(Yeast)
EUR 1030

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase D (cyp12)
MBS1205200-01mgEColi 0.1mg(E-Coli)
EUR 1085

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase D (cyp12)
MBS1205200-01mgYeast 0.1mg(Yeast)
EUR 1170

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase E (cyp10)
MBS1176479-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1195

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase E (cyp10)
MBS1176479-002mgEColi 0.02mg(E-Coli)
EUR 840

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase E (cyp10)
MBS1176479-002mgYeast 0.02mg(Yeast)
EUR 985

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase E (cyp10)
MBS1176479-01mgEColi 0.1mg(E-Coli)
EUR 1010

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase E (cyp10)
MBS1176479-01mgYeast 0.1mg(Yeast)
EUR 1120

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A2 (cyp1)
MBS1121114-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1080

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A2 (cyp1)
MBS1121114-002mgEColi 0.02mg(E-Coli)
EUR 675

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A2 (cyp1)
MBS1121114-002mgYeast 0.02mg(Yeast)
EUR 835

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A2 (cyp1)
MBS1121114-01mgEColi 0.1mg(E-Coli)
EUR 780

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A2 (cyp1)
MBS1121114-01mgYeast 0.1mg(Yeast)
EUR 975

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A1 (cyp2)
MBS1006719-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1085

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A1 (cyp2)
MBS1006719-002mgEColi 0.02mg(E-Coli)
EUR 675

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A1 (cyp2)
MBS1006719-002mgYeast 0.02mg(Yeast)
EUR 840

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A1 (cyp2)
MBS1006719-01mgEColi 0.1mg(E-Coli)
EUR 785

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase A1 (cyp2)
MBS1006719-01mgYeast 0.1mg(Yeast)
EUR 980

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B2 (cyp9)
MBS1075856-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1105

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B2 (cyp9)
MBS1075856-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B2 (cyp9)
MBS1075856-002mgYeast 0.02mg(Yeast)
EUR 855

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B2 (cyp9)
MBS1075856-01mgEColi 0.1mg(E-Coli)
EUR 815

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase B2 (cyp9)
MBS1075856-01mgYeast 0.1mg(Yeast)
EUR 1005

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase H (cyp7)
MBS1036725-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1100

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase H (cyp7)
MBS1036725-002mgEColi 0.02mg(E-Coli)
EUR 695

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase H (cyp7)
MBS1036725-002mgYeast 0.02mg(Yeast)
EUR 850

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase H (cyp7)
MBS1036725-01mgEColi 0.1mg(E-Coli)
EUR 805

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase H (cyp7)
MBS1036725-01mgYeast 0.1mg(Yeast)
EUR 995

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp6 (cyp6)
MBS1159579-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1095

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp6 (cyp6)
MBS1159579-002mgEColi 0.02mg(E-Coli)
EUR 690

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp6 (cyp6)
MBS1159579-002mgYeast 0.02mg(Yeast)
EUR 850

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp6 (cyp6)
MBS1159579-01mgEColi 0.1mg(E-Coli)
EUR 805

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp6 (cyp6)
MBS1159579-01mgYeast 0.1mg(Yeast)
EUR 995

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp5 (cyp5)
MBS1129945-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1095

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp5 (cyp5)
MBS1129945-002mgEColi 0.02mg(E-Coli)
EUR 690

Recombinant Rhizopus oryzae Peptidyl-prolyl cis-trans isomerase cyp5 (cyp5)
MBS1129945-002mgYeast 0.02mg(Yeast)
EUR 850

Induction of cell cycle arrest and apoptosis by tomentosin in hepatocellular carcinoma HepG2 and Huh7 cells.

Tomentosin, a sesquiterpene lactone, is known to possess various biological activities. However, its anticarcinogenic activity against human hepatocellular carcinoma (HCC) cells has not been investigated in detail. Thus, this study aimed to elucidate the cytotoxic mechanism of tomentosin in human HCC cell lines HepG2 and Huh7.

 

WST-1, cell counting, and colony formation assay results showed that treatment with tomentosin decreased the viability and suppressed the proliferation rate of HepG2 and Huh7 cells in a dose- and time-dependent manner. Cell cycle analysis revealed increased population of cells at the SubG1 and G2/M stage, and decreased population of cells at the G0/1 stage in HepG2 and Huh7 cells treated with tomentosin.

 

Annexin V/propidium iodide double staining and TUNEL assay results showed increased apoptotic cell population and DNA fragmentation in HepG2 and Huh7 cells treated with tomentosin.

 

Western blotting analysis results showed that tomentosin treatment significantly increased the expression level of Bax, Bim (short form), cleaved PARP1, FOXO3, p53, pSer15p53, pSer20p53, pSer46p53, p21, and p27, but decreased the expression of Bcl2, caspase3, caspase7, caspase9, cyclin-dependent kinase 2 (CDK2), CDK4, CDK6, cyclinB1, cyclinD1, cyclinD2, cyclinD3, and cyclinE in a dose-dependent manner.

 

Taken together, this study revealed that tomentosin, which acted through cell cycle arrest and apoptosis, may be a useful therapeutic option against HCC.

Keywords: FOXO3; Tomentosin; apoptosis; cell cycle arrest; hepatocellular carcinoma; p53.

 

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
MBS1459505-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1125

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
MBS1459505-002mgEColi 0.02mg(E-Coli)
EUR 500

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
MBS1459505-002mgYeast 0.02mg(Yeast)
EUR 420

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
MBS1459505-01mgEColi 0.1mg(E-Coli)
EUR 750

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
MBS1459505-01mgYeast 0.1mg(Yeast)
EUR 695

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
RPC27910-100ug 100ug
EUR 863.2

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
RPC27910-1mg 1mg
EUR 3462

Recombinant Fusarium solani subsp. cucurbitae Cutinase (CUTA)
RPC27910-20ug 20ug
EUR 479.6

Recombinant Fusarium solani subsp. pisi Cutinase 2 (CUT2)
MBS1293837-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1135

Recombinant Fusarium solani subsp. pisi Cutinase 2 (CUT2)
MBS1293837-002mgEColi 0.02mg(E-Coli)
EUR 720

Recombinant Fusarium solani subsp. pisi Cutinase 2 (CUT2)
MBS1293837-002mgYeast 0.02mg(Yeast)
EUR 885

Recombinant Fusarium solani subsp. pisi Cutinase 2 (CUT2)
MBS1293837-01mgEColi 0.1mg(E-Coli)
EUR 860

Recombinant Fusarium solani subsp. pisi Cutinase 2 (CUT2)
MBS1293837-01mgYeast 0.1mg(Yeast)
EUR 1040

Recombinant Fusarium solani subsp. pisi Cutinase 3 (CUT3)
MBS1309358-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1135

Recombinant Fusarium solani subsp. pisi Cutinase 3 (CUT3)
MBS1309358-002mgEColi 0.02mg(E-Coli)
EUR 720

Recombinant Fusarium solani subsp. pisi Cutinase 3 (CUT3)
MBS1309358-002mgYeast 0.02mg(Yeast)
EUR 885

Recombinant Fusarium solani subsp. pisi Cutinase 3 (CUT3)
MBS1309358-01mgEColi 0.1mg(E-Coli)
EUR 860

Recombinant Fusarium solani subsp. pisi Cutinase 3 (CUT3)
MBS1309358-01mgYeast 0.1mg(Yeast)
EUR 1040

Recombinant Fusarium solani subsp. pisi Cutinase 1 (CUT1)
MBS1006051-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1120

Recombinant Fusarium solani subsp. pisi Cutinase 1 (CUT1)
MBS1006051-002mgEColi 0.02mg(E-Coli)
EUR 700

Recombinant Fusarium solani subsp. pisi Cutinase 1 (CUT1)
MBS1006051-002mgYeast 0.02mg(Yeast)
EUR 870

Recombinant Fusarium solani subsp. pisi Cutinase 1 (CUT1)
MBS1006051-01mgEColi 0.1mg(E-Coli)
EUR 840

Recombinant Fusarium solani subsp. pisi Cutinase 1 (CUT1)
MBS1006051-01mgYeast 0.1mg(Yeast)
EUR 1020

Recombinant Fusarium solani subsp. pisi Allergen Fus s I3596*
MBS1152901-005mgBaculovirus 0.05mg(Baculovirus)
EUR 870

Recombinant Fusarium solani subsp. pisi Allergen Fus s I3596*
MBS1152901-005mgEColi 0.05mg(E-Coli)
EUR 485

Recombinant Fusarium solani subsp. pisi Allergen Fus s I3596*
MBS1152901-005mgYeast 0.05mg(Yeast)
EUR 705

Recombinant Fusarium solani subsp. pisi Allergen Fus s I3596*
MBS1152901-02mgEColi 0.2mg(E-Coli)
EUR 635

Recombinant Fusarium solani subsp. pisi Allergen Fus s I3596*
MBS1152901-05mgEColi 0.5mg(E-Coli)
EUR 695

Recombinant Fusarium solani subsp. pisi D-amino-acid oxidase
MBS1086214-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1235

Recombinant Fusarium solani subsp. pisi D-amino-acid oxidase
MBS1086214-002mgEColi 0.02mg(E-Coli)
EUR 900

Recombinant Fusarium solani subsp. pisi D-amino-acid oxidase
MBS1086214-002mgYeast 0.02mg(Yeast)
EUR 1025

Recombinant Fusarium solani subsp. pisi D-amino-acid oxidase
MBS1086214-01mgEColi 0.1mg(E-Coli)
EUR 1080

Recombinant Fusarium solani subsp. pisi D-amino-acid oxidase
MBS1086214-01mgYeast 0.1mg(Yeast)
EUR 1170

Recombinant Fusarium solani subsp. phaseoli Glutamine synthetase (GLN1)
MBS1456389-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1230

Recombinant Fusarium solani subsp. phaseoli Glutamine synthetase (GLN1)
MBS1456389-002mgEColi 0.02mg(E-Coli)
EUR 890

Recombinant Fusarium solani subsp. phaseoli Glutamine synthetase (GLN1)
MBS1456389-002mgYeast 0.02mg(Yeast)
EUR 1020

Recombinant Fusarium solani subsp. phaseoli Glutamine synthetase (GLN1)
MBS1456389-01mgEColi 0.1mg(E-Coli)
EUR 1070

Recombinant Fusarium solani subsp. phaseoli Glutamine synthetase (GLN1)
MBS1456389-01mgYeast 0.1mg(Yeast)
EUR 1160

Recombinant Fusarium solani subsp. pisi Pisatin demethylase (PDAT9), partial
MBS1295150-INQUIRE INQUIRE Ask for price

Recombinant Fusarium solani subsp. phaseoli Thiamine thiazole synthase (sti35)
MBS1141449-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1200

Recombinant Fusarium solani subsp. phaseoli Thiamine thiazole synthase (sti35)
MBS1141449-002mgEColi 0.02mg(E-Coli)
EUR 855

Recombinant Fusarium solani subsp. phaseoli Thiamine thiazole synthase (sti35)
MBS1141449-002mgYeast 0.02mg(Yeast)
EUR 990

Recombinant Fusarium solani subsp. phaseoli Thiamine thiazole synthase (sti35)
MBS1141449-01mgEColi 0.1mg(E-Coli)
EUR 1025

Recombinant Fusarium solani subsp. phaseoli Thiamine thiazole synthase (sti35)
MBS1141449-01mgYeast 0.1mg(Yeast)
EUR 1125

Recombinant Fusarium solani subsp. pisi Pisatin demethylase (PDA6-1), partial
MBS1234241-INQUIRE INQUIRE Ask for price

Recombinant Fusarium solani subsp. pisi Dynein heavy chain, cytoplasmic (DHC1), partial
MBS1101783-INQUIRE INQUIRE Ask for price

Recombinant Fusarium solani subsp. pisi Cutinase transcription factor 1 beta (CTF1-BETA), partial
MBS1191281-INQUIRE INQUIRE Ask for price

Recombinant Fusarium solani subsp. pisi Cutinase transcription factor 1 alpha (CTF1-ALPHA), partial
MBS1011253-INQUIRE INQUIRE Ask for price

Fusarium sp.
PCR-VH007-PCRVH00748D PCR-VH007-48D
EUR 230

Fusarium sp.
PCR-VH007-PCRVH00796D PCR-VH007-96D
EUR 312

Fusarium sp.
Oneq-VH007-OneqVH007100D Oneq-VH007-100D
EUR 515

Fusarium sp.
Oneq-VH007-OneqVH007150D Oneq-VH007-150D
EUR 594

Fusarium sp.
Oneq-VH007-OneqVH00750D Oneq-VH007-50D
EUR 413

Fusarium sp. PCR kit
PCR-VH007-48D 50T
EUR 543.6

Fusarium sp. PCR kit
PCR-VH007-96D 100T
EUR 686.4

Fusarium sp. RT PCR kit
RTq-VH007-100D 100T
EUR 860.4

Fusarium sp. RT PCR kit
RTq-VH007-150D 150T
EUR 969.6

Fusarium sp. RT PCR kit
RTq-VH007-50D 50T
EUR 717.6

Fusarium antigen ELISA Kit
DEIABL66 96T
EUR 704
Description: Soil, growing or harvested plants, grain, food, washes from industrial equipment and other types of material

Fusarium sp. One-Step PCR kit
Oneq-VH007-100D 100T
EUR 1039.2

Fusarium sp. One-Step PCR kit
Oneq-VH007-150D 150T
EUR 1177.2

Fusarium sp. One-Step PCR kit
Oneq-VH007-50D 50T
EUR 861.6

Recombinant Fusarium culmorum Alkaline proteinase
MBS1154415-002mgBaculovirus 0.02mg(Baculovirus)
EUR 985

Recombinant Fusarium culmorum Alkaline proteinase
MBS1154415-002mgEColi 0.02mg(E-Coli)
EUR 550

Recombinant Fusarium culmorum Alkaline proteinase
MBS1154415-002mgYeast 0.02mg(Yeast)
EUR 740

Recombinant Fusarium culmorum Alkaline proteinase
MBS1154415-01mgEColi 0.1mg(E-Coli)
EUR 655

Recombinant Fusarium culmorum Alkaline proteinase
MBS1154415-01mgYeast 0.1mg(Yeast)
EUR 865

Recombinant Fusarium culmorum Allergen Fus c 3
MBS1472273-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1320

Recombinant Fusarium culmorum Allergen Fus c 3
MBS1472273-002mgEColi 0.02mg(E-Coli)
EUR 1005

Recombinant Fusarium culmorum Allergen Fus c 3
MBS1472273-002mgYeast 0.02mg(Yeast)
EUR 1110

Recombinant Fusarium culmorum Allergen Fus c 3
MBS1472273-01mgEColi 0.1mg(E-Coli)
EUR 1175

Recombinant Fusarium culmorum Allergen Fus c 3
MBS1472273-01mgYeast 0.1mg(Yeast)
EUR 1265

Fusarium Species TaqProbe qPCR Detection; 100 RXN
TBS42019-100 100 tests
EUR 398

Recombinant Fusarium poae Trichodiene synthase (TRI5)
MBS1114717-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Fusarium poae Trichodiene synthase (TRI5)
MBS1114717-002mgEColi 0.02mg(E-Coli)
EUR 915

Recombinant Fusarium poae Trichodiene synthase (TRI5)
MBS1114717-002mgYeast 0.02mg(Yeast)
EUR 1045

Recombinant Fusarium poae Trichodiene synthase (TRI5)
MBS1114717-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Fusarium poae Trichodiene synthase (TRI5)
MBS1114717-01mgYeast 0.1mg(Yeast)
EUR 1185

Recombinant Fusarium oxysporum Endoglucanase type C
MBS1171975-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1285

Recombinant Fusarium oxysporum Endoglucanase type C
MBS1171975-002mgEColi 0.02mg(E-Coli)
EUR 960

Recombinant Fusarium oxysporum Endoglucanase type C
MBS1171975-002mgYeast 0.02mg(Yeast)
EUR 1075

Recombinant Fusarium oxysporum Endoglucanase type C
MBS1171975-01mgEColi 0.1mg(E-Coli)
EUR 1120

Recombinant Fusarium oxysporum Endoglucanase type C
MBS1171975-01mgYeast 0.1mg(Yeast)
EUR 1225

Rabbit anti-Fusarium oxysporum (Fusarium vascular wilt) CYP505 Polyclonal Antibody
MBS9015602-INQUIRE INQUIRE Ask for price

Rabbit anti-Fusarium oxysporum (Fusarium vascular wilt) CYP55A1 Polyclonal Antibody
MBS9016807-INQUIRE INQUIRE Ask for price

Recombinant Fusarium culmorum Trichodiene synthase (TRI5)
MBS1457825-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1250

Recombinant Fusarium culmorum Trichodiene synthase (TRI5)
MBS1457825-002mgEColi 0.02mg(E-Coli)
EUR 915

Recombinant Fusarium culmorum Trichodiene synthase (TRI5)
MBS1457825-002mgYeast 0.02mg(Yeast)
EUR 1040

Recombinant Fusarium culmorum Trichodiene synthase (TRI5)
MBS1457825-01mgEColi 0.1mg(E-Coli)
EUR 1100

Recombinant Fusarium culmorum Trichodiene synthase (TRI5)
MBS1457825-01mgYeast 0.1mg(Yeast)
EUR 1185

Recombinant Fusarium culmorum Thioredoxin-like protein
MBS1424912-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1045
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