Chromatin – DNA fragments by sonication and immunoprecipitated
Regulatory mechanisms governing chromatin organization and function

Regulatory mechanisms governing chromatin organization and function

Zoe
Zoe

Nucleosomes, the fundamental constructions used to bundle genetic info into chromatin, are topic to a various array of chemical modifications. Numerous these marks function interplay hubs for a lot of nuclear proteins and supply essential structural options for protein recruitment. Dynamic deposition and elimination of chromatin modifications by regulatory proteins guarantee their right deposition to the genome, which is important for DNA replication, transcription, chromatin compaction, or DNA injury restore. The spatiotemporal regulation and upkeep of chromatin marks depends on coordinated actions of author, eraser, and reader enzymes and infrequently depends upon complicated multicomponent regulatory circuits. In recent times, the sphere has made huge advances in uncovering the mechanisms that regulate chromatin modifications.

 

Right here, we focus on well-established and rising ideas in chromatin biology starting from cooperativity and multivalent interactions to regulatory suggestions loops and elevated native focus of chromatin-modifying enzymes.  The Reworking Progress Issue-β (TGFβ) signaling pathway controls transcription by regulating enhancer exercise. How TGFβ-regulated enhancers are chosen and what chromatin adjustments are related to TGFβ-dependent enhancers regulation are nonetheless unclear. Right here we report that TGFβ therapy triggers quick and widespread improve in chromatin accessibility in about 80% of the enhancers of regular mouse mammary epithelial-gland cells, regardless of whether or not they’re activated, repressed or not regulated by TGFβ. This enhancer opening depends upon each the canonical and non-canonical TGFβ pathways. Most TGFβ-regulated genes are situated round enhancers regulated in the identical method, typically creating domains of a number of co-regulated genes that we time period TGFβ regulatory domains (TRD).

 

Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology

Utilizing Hello-C, promoter-capture Hello-C (pCHi-C), and different genome-wide approaches in skeletal muscle progenitors that inducibly categorical a grasp transcription issue, Pax7, we systematically characterize at high-resolution the spatio-temporal re-organization of compartments and promoter-anchored interactions as a consequence of myogenic dedication and differentiation. We determine key promoter-enhancer interplay motifs, particularly, cliques and networks, and interactions which might be depending on Pax7 binding. Remarkably, Pax7 binds to a majority of super-enhancers, and along with a cadre of interacting transcription elements, assembles feed-forward regulatory loops.
Throughout differentiation, epigenetic reminiscence and chronic looping are maintained at a subset of Pax7 enhancers within the absence of Pax7. We additionally determine and functionally validate a beforehand uncharacterized Pax7-bound enhancer hub that regulates the important myosin heavy chain cluster throughout skeletal muscle cell differentiation. Our research lay the groundwork for understanding the function of Pax7 in orchestrating adjustments within the three-dimensional chromatin conformation in muscle progenitors. Class I histone deacetylase complexes play important roles in lots of nuclear processes. While they comprise a standard catalytic subunit, they’ve various modes of motion decided by related elements within the distinct complexes.
The deacetylase module from the NuRD complicated comprises three protein domains that management the recruitment of chromatin to the deacetylase enzyme, HDAC1/2. Utilizing biochemical approaches and cryo-electron microscopy, we have now decided how three chromatin-binding domains (MTA1-BAH, MBD2/three and RBBP4/7) are assembled in relation to the core complicated in order to facilitate interplay of the complicated with the genome. We observe a placing association of the BAH domains suggesting a possible mechanism for binding to di-nucleosomes.
 Regulatory mechanisms governing chromatin organization and function
Regulatory mechanisms governing chromatin organization and function

The X-ray crystal structure of the N-terminal domain of Ssr4, a Schizosaccharomyces pombe chromatin-remodelling protein

Ssr4 is a yeast protein from Schizosaccharomyces pombe and is a necessary a part of the chromatin-remodelling [SWI/SNF and RSC (remodelling the structure of chromatin)] complexes present in S. pombe. These complexes (or their homologues) regulate gene expression in eukaryotic organisms, affecting a lot of genes each positively and negatively. The downstream results are seen in growth, and in people have implications for illness similar to most cancers. The chromatin construction is altered by modifying the DNA-histone contacts, thus opening up or closing down sections of DNA to particular transcription elements that regulate the transcription of genes. The Ssr4 sequence has little homology to different sequences within the Protein Knowledge Financial institution, so the construction was solved utilizing an iodine spinoff with SAD phasing.
The construction of the N-terminal area is an antiparallel β-sheet of seven strands with α-helices on one facet and random coil on the opposite. The construction is considerably completely different to deposited constructions and was used as a goal in the newest Important Evaluation of Methods for Protein Construction Prediction Rising proof means that chromodomain-helicase-DNA-binding (CHD) proteins are concerned in stem cell upkeep and differentiation through the coordination of chromatin construction and gene expression.

PPARγ phosphorylation inhibitor 1

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Description: PPARγ phosphorylation inhibitor 1

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Description: PPARγ phosphorylation inhibitor 1

PPARγ phosphorylation inhibitor 1

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Description: PPARγ phosphorylation inhibitor 1

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Description: PPARγ phosphorylation inhibitor 1

PPARγ phosphorylation inhibitor 1

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Description: PPARγ phosphorylation inhibitor 1

Channel Phosphorylation Sampler Kit

E051011 5x50ug
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Apoptosis Phosphorylation Sampler Kit

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (Dyrk1a) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Antibody

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abx431220-200l 200 µl
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EnzyFluo AMPK Phosphorylation Assay Kit

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Calcium-Binding Tyrosine Phosphorylation-Regulated Protein (CABYR) Antibody

20-abx111304
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Calcium-Binding Tyrosine Phosphorylation-Regulated Protein (CABYR) Antibody

20-abx148788
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abx231170-100ug 100 ug
EUR 610.8

Calcium-Binding Tyrosine Phosphorylation-Regulated Protein (CABYR) Antibody

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Cell Motility Phosphorylation Sampler Kit

E051010 5x50ug
EUR 700
Description: Available in various conjugation types.

Growth and Differentiation Phosphorylation

E051014 5x50ug
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Description: Available in various conjugation types.

Rat phosphorylation AKT(p-AKT)ELISA Kit

SL1158Ra -
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YLA0398RA-48T 48T Ask for price

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Chemical Phosphorylation Reagent I (CPR I)

6001-1g 1 g
EUR 558
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Human phosphorylation AKT(p-AKT)ELISA Kit

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EUR 433.2

Rat phosphorylation AKT, p-AKT GENLISA ELISA

KLR1201 1 x 96 wells
EUR 341

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody

CAU21784-100ul 100ul
EUR 235.2

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Human phosphorylation AKT, p-AKT GENLISA ELISA

KBH4054 1 x 96 wells
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Polyclonal Antibody to Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A)

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EUR 245

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human)

4-PAJ193Hu01
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A)

EnzyFluo Human/Mouse RPS6(S235/236) Phosphorylation ELISA

ERPS6-100 100
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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human), APC

4-PAJ193Hu01-APC
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). This antibody is labeled with APC.

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human), Cy3

4-PAJ193Hu01-Cy3
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). This antibody is labeled with Cy3.

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human), HRP

4-PAJ193Hu01-HRP
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). This antibody is labeled with HRP.

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human), PE

4-PAJ193Hu01-PE
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). This antibody is labeled with PE.

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EpiQuik In Situ Histone H3 Phosphorylation (Ser10) Assay Kit 

P-7001
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  • 96 Assays
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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human), FITC

4-PAJ193Hu01-FITC
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). This antibody is labeled with FITC.

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human), APC-Cy7

4-PAJ193Hu01-APC-Cy7
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). This antibody is labeled with APC-Cy7.

Rabbit Polyclonal antibody to Dyrk3 (dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3)

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Rat Phosphorylation of AMP activated protein kinase ELISA kit

E02P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Phosphorylation of AMP activated protein kinase ELISA kit

E02P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Phosphorylation of AMP activated protein kinase ELISA kit

E02P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Phosphorylation of AMP activated protein kinase ELISA kit

E07P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Phosphorylation of AMP activated protein kinase ELISA kit

E07P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Phosphorylation of AMP activated protein kinase ELISA kit

E07P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Phosphorylation of AMP activated protein kinase ELISA kit

E08P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Phosphorylation of AMP activated protein kinase ELISA kit

E08P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Phosphorylation of AMP activated protein kinase ELISA kit

E08P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Phosphorylation of AMP activated protein kinase ELISA kit

E06P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Phosphorylation of AMP activated protein kinase ELISA kit

E06P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Phosphorylation of AMP activated protein kinase ELISA kit

E06P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Dual specificity tyrosine- phosphorylation- regulated kin

ELI-27714Ra 96 Tests
EUR 1113.6

Mouse Phosphorylation of AMP activated protein kinase ELISA kit

E03P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Phosphorylation of AMP activated protein kinase ELISA kit

E03P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Phosphorylation of AMP activated protein kinase ELISA kit

E03P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Phosphorylation of AMP activated protein kinase ELISA kit

E01P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Phosphorylation of AMP activated protein kinase ELISA kit

E01P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Phosphorylation of AMP activated protein kinase ELISA kit

E01P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Cabyr ELISA Kit| Mouse Calcium-binding tyrosine phosphorylation

EF014364 96 Tests
EUR 826.8

Rabbit Phosphorylation of AMP activated protein kinase ELISA kit

E04P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Phosphorylation of AMP activated protein kinase ELISA kit

E04P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Phosphorylation of AMP activated protein kinase ELISA kit

E04P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Phosphorylation of AMP activated protein kinase ELISA kit

E09P0187-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Phosphorylation of AMP activated protein kinase ELISA kit

E09P0187-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Phosphorylation of AMP activated protein kinase ELISA kit

E09P0187-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Phosphorylation of AMP activated protein kinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Fish phosphorylation of AMP-activated protein kinase ELISA Kit

QY-E150009 96T
EUR 573.6

Rat Phosphorylation Mitogen activated Protein Kinase 14 ELISA kit

E01A16132 96T
EUR 700
Description: ELISA

Rat Phosphorylation Mitogen activated Protein Kinase 9 ELISA kit

E01A16133 96T
EUR 700
Description: ELISA

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) Polyclonal Antibody (Human), Biotinylated

4-PAJ193Hu01-Biotin
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). This antibody is labeled with Biotin.

Goat Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

E01A50481 96T
EUR 700
Description: ELISA

Goat Phosphorylation Mitogen activated Protein Kinase 14 ELISA kit

E01A51035 96T
EUR 700
Description: ELISA

Goat Phosphorylation Mitogen activated Protein Kinase 9 ELISA kit

E01A51036 96T
EUR 700
Description: ELISA

Human Phosphorylation Mitogen activated Protein Kinase 14 ELISA kit

E01A7387 96T
EUR 700
Description: ELISA
Nonetheless, the molecular perform of some CHD proteins in stem cell regulation continues to be poorly understood. Herein, we present that Chd9 knockdown (KD) in mouse embryonic stem cells (ESCs) cultured in regular serum media, not in 2i-leukemia inhibitory issue (LIF) media, causes speedy cell proliferation.
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